Wahnschaffe U
Medizinische Hochschule Hannover, Institut für Experimentelle Pathologie, F.R.G.
Exp Pathol. 1988;34(2):79-84. doi: 10.1016/s0232-1513(88)80032-x.
Treatment of cultured cells with tannic acid after fixation with glutaraldehyde and osmification prevents the ultrastructural deterioration of cellular structures during dehydration and impregnation. Membranes of tannic acid-treated cells showed an increased contrast and sharp delineation of the polar zone. These results are discussed on the basis of simultaneous interactions of tannic acid with saturated phospholipids and OSO4. No ultrastructural differences are found between cells treated with tannic acids of different molecular weights.
用戊二醛固定并经锇酸处理后，用单宁酸处理培养细胞可防止脱水和浸透过程中细胞结构的超微结构恶化。单宁酸处理的细胞的膜显示出对比度增加，极性区轮廓清晰。基于单宁酸与饱和磷脂和四氧化锇的同时相互作用对这些结果进行了讨论。不同分子量的单宁酸处理的细胞之间未发现超微结构差异。
