Persi M A, Burnham J C
Sabouraudia. 1981 Mar;19(1):1-8. doi: 10.1080/00362178185380021.
Blastospores of Candida albicans, grown in YM broth (Difco, pH 6.0), were treated with tannic acid as part of a fixation schedule with glutaraldehyde and osmium tetroxide. The results obtained were compared to routine fixation procedures employing glutaraldehyde-osmium tetroxide and potassium permanganate, as well as several schedules using various combinations of the aforementioned fixatives. Glutaraldehyde-osmium tetroxide-tannic acid fixed cells had several morphological structures accentuated, including sharply delineated cytoplasmic and organelle membranes, a detailed cytoplasmic matrix, and an intensified layered cell wall. Tannic acid has been found to react as a mordant between osmium tetroxide and lead citrate, forming a "stabilizing matrix" within the cells. Blastospores appeared to have an improved comprehensive ultrastructural appearance, a result of the tannic acid-matrix preserving cellular components for electron microscopy.
白色念珠菌的芽生孢子在YM肉汤（Difco，pH 6.0）中培养，作为用戊二醛和四氧化锇固定程序的一部分，用单宁酸处理。将获得的结果与采用戊二醛-四氧化锇和高锰酸钾的常规固定程序以及使用上述固定剂各种组合的几种固定方案进行比较。戊二醛-四氧化锇-单宁酸固定的细胞有几种形态结构得到增强，包括清晰界定的细胞质和细胞器膜、详细的细胞质基质以及强化的分层细胞壁。已发现单宁酸作为四氧化锇和柠檬酸铅之间的媒染剂起反应，在细胞内形成“稳定基质”。芽生孢子似乎具有改善的综合超微结构外观，这是单宁酸基质为电子显微镜保留细胞成分的结果。
