College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, China.
Department of Chemistry and Chemical Engineering, Inha University, Incheon, South Korea.
Phytochem Anal. 2020 Mar;31(2):242-251. doi: 10.1002/pca.2888. Epub 2019 Aug 21.
Ephedrine is a typical compound found in lots of plant species that is used in several medicines for the treatment of asthma and bronchitis. However, excess amounts are harmful to humans, so it needs to be removed.
This study developed a multi-phase extraction (MPE) method with a molecular imprinted polymer (MIP) coated ionic liquid (IL)-based silica (SiO @IL@MIP) to simultaneously extract and separate ephedrine from Pinellia ternata, 10 medicines, and urine samples.
IL was immobilized on silica. Subsequently, the IL was combined with the functional monomer, followed by the addition of the crosslinker and template. The resulting sorbent was applied to the MPE, and the extraction, washing and elution solvents were evaluated.
Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) confirmed the synthesis of SiO @IL@MIP. A maximum adsorption amount of 5.76 mg/g was obtained at 30°C at a neutral pH. In MPE, 10.00 mL of methanol could extract all the ephedrine from Pinellia ternata. The interference was removed by washing with 4.00 mL of water, ethanol, and acetonitrile. Finally, 8.00 mL of methanol/acetic acid (99:1, v/v) was applied as the elution solvent. The following were extracted: 5.50 μg/g of ephedrine from Pinellia ternata, 0.00-46.50 μg/g from the 10 herbal medicines, and 68.70-102.80 μg/mL in the urine samples.
The proposed method was applied successfully to the simultaneously extraction and separation of ephedrine from plants and medicines. These results are expected to provide important data for the development of new methods for the separation and purification of bioactive compounds.
麻黄碱是许多植物物种中常见的典型化合物，用于治疗哮喘和支气管炎等多种药物。然而，过量对人体有害，因此需要去除。
本研究开发了一种多相萃取（MPE）方法，使用分子印迹聚合物（MIP）涂覆的离子液体（IL）-基于二氧化硅（SiO@IL@MIP）从半夏、10 种药物和尿液样品中同时提取和分离麻黄碱。
IL 固定在二氧化硅上。随后，IL 与功能单体结合，然后加入交联剂和模板。所得吸附剂用于 MPE，并评估萃取、洗涤和洗脱溶剂。
傅里叶变换红外（FTIR）光谱和扫描电子显微镜（SEM）证实了 SiO@IL@MIP 的合成。在中性 pH 值为 30°C 时，最大吸附量为 5.76mg/g。在 MPE 中，10.00mL 甲醇可以从半夏中提取所有麻黄碱。通过用 4.00mL 水、乙醇和乙腈洗涤可以去除干扰。最后，8.00mL 甲醇/乙酸（99:1，v/v）作为洗脱溶剂。从半夏中提取出 5.50μg/g 的麻黄碱、10 种草药中 0.00-46.50μg/g 的麻黄碱和尿液样本中 68.70-102.80μg/mL 的麻黄碱。
该方法成功应用于从植物和药物中同时提取和分离麻黄碱。这些结果有望为生物活性化合物的分离和纯化新方法的开发提供重要数据。
