College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, P. R. China.
Department of Chemistry and Chemical Engineering, Inha University, Incheon, Korea.
J Sep Sci. 2019 Oct;42(19):3047-3053. doi: 10.1002/jssc.201900554. Epub 2019 Jul 31.
Aristolochic acid I is a toxic compound found in the genus of Aristolochia plants, which are commonly used as herbal cough treatment medicines. To remove the aristolochic acid I in extract efficiently and selectively, a molecularly imprinted polymer composed of ethylimidazole ionic liquid-based zeolitic imidazolate framework-67 was synthesized and used as the adsorbent. Under the conditions optimized by the software design expert, the sorbent showed highest adsorption amount of 34.25 mg/g in methanol/water (95:5, v/v) at 39°C for 138 min. The sorbent was then applied to solid phase extraction to isolate aristolochic acid I from the extract of the herbal plant Fibraurea Recisa Pierre. 0.043 mg/g of aristolochic acid I was obtained after the loading, washing, and elution processes. The limit of detection of 2.41 × 10 mg/mL and good recoveries provided evidence for the accuracy of this method.
马兜铃酸 I 是一种存在于马兜铃属植物中的有毒化合物，这些植物通常被用作草药止咳药物。为了有效地、选择性地去除提取物中的马兜铃酸 I，合成了一种由乙基咪唑离子液体基沸石咪唑酯骨架-67 组成的分子印迹聚合物，并将其用作吸附剂。在软件设计专家优化的条件下，吸附剂在 39°C 下于甲醇/水（95:5，v/v）中吸附 138 分钟时表现出最高的吸附量 34.25mg/g。然后将该吸附剂应用于固相萃取，从草药 Fibraurea Recisa Pierre 的提取物中分离出马兜铃酸 I。在加载、洗涤和洗脱过程后，得到 0.043mg/g 的马兜铃酸 I。检测限为 2.41×10 mg/mL，回收率良好，为该方法的准确性提供了证据。
