Department of Reptiles and Amphibians, Clinic for Small Mammals, Reptiles and Birds, University of Veterinary Medicine, Hannover, Germany.
Institute for Anatomy, University of Veterinary Medicine, Hannover, Germany, University of Veterinary Medicine Hannover, Hannover, Germany.
PLoS One. 2019 Aug 22;14(8):e0221050. doi: 10.1371/journal.pone.0221050. eCollection 2019.
The aim of this study was to describe the specific gross and radiographic anatomy of the digestive tract of inland bearded dragons (Pogona vitticeps). Eleven bearded dragon cadavers of both sexes (6 females, 5 males) were dissected to examine, measure, and document the specific gross anatomy of the alimentary canal. Measurements collected from the cadavers included snout-vent length, total length of the alimentary canal, and the lengths of the individual sections of the gastrointestinal tract, including the esophagus, stomach, small intestine, ampulla coli, isthmus coli, rectum, and the distance from the coprodeum to the vent opening. Twenty-two healthy adult bearded dragons (13 females, 9 males) maintained under standardized husbandry conditions underwent a physical examination, blood collection, and whole-body dorsoventral and lateral survey radiographs; these animals were used to provide the radiographic images of the complete digestive tract. For the subsequent contrast passage studies, two different contrast media, barium sulfate (BaSO4, Barilux suspension) and an iodinated ionic radiocontrast agent (Sodium meglumine amidotrizoate [SMAT], Gastrografin), were used. Water-diluted Barilux suspension (dose 9 ml/kg) was administered orally to 5 bearded dragons, while Gastrografin (dose 5ml/kg) was administered orally to 21 bearded dragons. Four animals were used for both contrast media studies, but received a break of four weeks in between. Dorsoventral and laterolateral radiographs were collected at 0 (baseline), 15, 30, and 45 minutes and 1, 2, 3, 4, 5, 6, 8, 10, 12, 24, 30, and 36 hours after each contrast medium was administered. Both contrast media were found to illustrate the alimentary tracts in the adult bearded dragons. Transit time was substantially faster with SMAT, and SMAT illustrated the entire gastrointestinal tract within 36 hours; BaSO4 did not fully illustrate the gastrointestinal tract in 36 hours. These results might serve as a guideline for the interpretation of subsequent contrast studies in this lizard species.
本研究旨在描述内陆鬃狮蜥（Pogona vitticeps）消化道的具体大体和放射学解剖结构。对 11 具雌雄两性的鬃狮蜥尸体（6 只雌性，5 只雄性）进行解剖，以检查、测量和记录消化道的具体大体解剖结构。从尸体中收集的测量值包括吻肛长、消化道总长度以及胃肠道各节段的长度，包括食管、胃、小肠、结肠壶腹、结肠峡部、直肠以及从直肠到泄殖腔开口的距离。22 只在标准化饲养条件下饲养的健康成年鬃狮蜥（13 只雌性，9 只雄性）接受了体格检查、血液采集和全身背腹位及侧位放射照相检查；这些动物被用来提供完整消化道的放射图像。随后的对比显影研究中，使用了两种不同的对比剂，硫酸钡（BaSO4，Barilux 混悬液）和碘离子型放射性对比剂（Sodium meglumine amidotrizoate [SMAT]，Gastrografin）。将水稀释的 Barilux 混悬液（剂量 9ml/kg）口服给予 5 只鬃狮蜥，而将 Gastrografin（剂量 5ml/kg）口服给予 21 只鬃狮蜥。有 4 只动物同时接受了两种对比剂的研究，但在两种对比剂之间间隔了 4 周。在口服对比剂后 0（基线）、15、30 和 45 分钟以及 1、2、3、4、5、6、8、10、12、24、30 和 36 小时采集背腹位和侧位放射照相。两种对比剂都能显示成年鬃狮蜥的消化道。SMAT 的通过时间明显更快，在 36 小时内即可显示整个胃肠道；BaSO4 在 36 小时内未能完全显示胃肠道。这些结果可能为该蜥蜴物种的后续对比研究的解释提供指导。
