抗铜绿假单胞菌氨基糖苷类耐药因子的单克隆抗体。

Monoclonal antibody to an aminoglycoside-resistance factor from Pseudomonas aeruginosa.

作者信息

Norris S A, Sciortino C V

机构信息

Department of Medicine, Medical University of South Charleston.

出版信息

J Infect Dis. 1988 Dec;158(6):1324-8. doi: 10.1093/infdis/158.6.1324.

DOI:10.1093/infdis/158.6.1324

PMID:3143766

Abstract

Aminoglycoside resistance (AMGr) in Pseudomonas aeruginosa is most commonly due to a membrane permeability factor. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins (OMPs) from AMGr strains of P. aeruginosa (resistant to tobramycin, gentamicin, and amikacin) revealed a unique OMP of 42 kilodaltons (kDa) when compared with aminoglycoside-susceptible strains of P. aeruginosa. A monoclonal antibody (MAb) to an AMGr strain of P. aeruginosa was prepared and used to study AMGr. This MAb, PS1, recognized a unique OMP of 42 kDa present primarily in AMGr strains. MAb PS1 had a binding sensitivity of 83% (n = 46), for AMGr P. aeruginosa, and a specificity of 85% (n = 36), as determined by immunoblot assay. This MAb may be useful for investigating the basis of AMGr in P. aeruginosa.

摘要

铜绿假单胞菌中的氨基糖苷类耐药性（AMGr）最常见的原因是一种膜通透性因子。对铜绿假单胞菌AMGr菌株（对妥布霉素、庆大霉素和阿米卡星耐药）的外膜蛋白（OMPs）进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，结果显示，与氨基糖苷类敏感的铜绿假单胞菌菌株相比，存在一种独特的42千道尔顿（kDa）的OMP。制备了一种针对铜绿假单胞菌AMGr菌株的单克隆抗体（MAb）并用于研究AMGr。这种MAb，即PS1，识别一种主要存在于AMGr菌株中的独特的42 kDa的OMP。通过免疫印迹分析确定，MAb PS1对AMGr铜绿假单胞菌的结合敏感性为83%（n = 46），特异性为85%（n = 36）。这种MAb可能有助于研究铜绿假单胞菌中AMGr的基础。