Karimi Poorya, Shafaghi-Sisi Soheila, Meamar Ahmad Reza, Nasiri Gelareh, Razmjou Elham
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Front Vet Sci. 2022 Jun 22;9:927185. doi: 10.3389/fvets.2022.927185. eCollection 2022.
and spp. are the most critical parasites common between humans and cats. The close association of cats with humans in urban areas persuaded us to investigate the prevalence of these parasites in stray and household cats and their possible role in the owners' infection. Herein, 132 and 33 fecal samples of stray and household cats, respectively, and 33 blood samples of their owners were collected in Tehran, Iran. The prevalence of was determined by targeting the gene in the feces of stray and household cats and the blood of cat owners. Furthermore, genotypes of were identified based on the multilocus genotyping of BTUB, GRA6, SAG3, and APICO loci. spp. were detected by targeting the second internal transcribed spacer (ITS-2) of the ribosomal DNA of these parasites in the cats' feces and the humans' blood. Also, IgG was assessed in the human serum samples. The gene amplification showed that 15.2% of stray cats, 18.2% of household cats, and 51.5% of cat owners were infected with . The multilocus sequence analysis revealed the predominance of genotype I of in stray cats and genotype II of in household cats and cat owners. The amplifying of ITS-2 revealed a high prevalence of infection (47.0%) in stray cats, whereas no infection was found in the feces of household cats or the serum of cat owners. Likewise, IgG was not detected in the serum of humans. The lower prevalence of in stray/household cats than in the cat owners indicates the limited impact of close contact with infected cats in human toxoplasmosis. However, the high prevalence of infection in stray cats can cause contamination of the environment by excreting eggs that may lead to infecting humans through soil or water. Therefore, public health education in urban management planning is necessary for routine urban cat deworming programs and for training the healthcare workers to prevent, control, and treat these infections.
[某种寄生虫名称]和[另一种寄生虫名称]是人类和猫之间最常见且最关键的寄生虫。猫在城市地区与人类的密切关联促使我们调查这些寄生虫在流浪猫和家猫中的感染率，以及它们在猫主人感染中的可能作用。在此，我们在伊朗德黑兰分别收集了132份流浪猫和33份家猫的粪便样本，以及33份猫主人的血液样本。通过针对流浪猫和家猫粪便以及猫主人血液中的[特定基因名称]基因来确定[某种寄生虫名称]的感染率。此外，基于BTUB、GRA6、SAG3和APICO基因座的多位点基因分型来鉴定[某种寄生虫名称]的基因型。通过针对这些寄生虫核糖体DNA的第二内部转录间隔区（ITS - 2）来检测猫粪便和人类血液中的[另一种寄生虫名称]。同时，在人类血清样本中评估[某种寄生虫抗体名称]IgG。[特定基因名称]基因扩增显示，15.2%的流浪猫、18.2%的家猫以及51.5%的猫主人感染了[某种寄生虫名称]。多位点序列分析揭示，流浪猫中[某种寄生虫名称]基因型I占主导，家猫和猫主人中[某种寄生虫名称]基因型II占主导。ITS - 2扩增显示流浪猫中[另一种寄生虫名称]感染率很高（47.0%），而在家猫粪便或猫主人血清中未发现感染。同样，在人类血清中未检测到[某种寄生虫抗体名称]IgG。流浪猫/家猫中[某种寄生虫名称]的感染率低于猫主人，这表明与感染猫密切接触对人类弓形虫病的影响有限。然而，流浪猫中[另一种寄生虫名称]的高感染率会通过排出虫卵污染环境，可能导致人类通过土壤或水感染。因此，在城市管理规划中进行公共卫生教育对于常规城市猫驱虫计划以及培训医护人员预防、控制和治疗这些感染是必要的。
