Animal Experimental Center, North Sichuan Medical College , Nanchong City , China.
Department of Histology and Embryology, North Sichuan Medical College , Nanchong City , China.
Pharm Biol. 2019 Dec;57(1):477-484. doi: 10.1080/13880209.2019.1645701.
In China, the herb Gagnep. (Fabaceae, ST) (Committee of National Pharmacopeia. 2015) exhibits anti-inflammatory, antitumor, and antiviral effects. However, to date, there have been few studies on its gastrointestinal effect. The gastrointestinal effect of the methanol extract of ST rhizome (STR) was evaluated. Study was conducted from February to December 2018. , antidiarrheal activity of STR (125, 250 and 500 mg/kg; orally) in castor oil-induced diarrheal mice was studied. , the effects of STR (0.01-10 mg/mL) on the isolated tissue preparations of rabbit jejunum were also investigated, the rabbit jejunum stripes were pre-contracted with Ach (10M), K (60 mM) and tested in the presence of STR, the possible spasmolytic effect was analyzed in the pretreatment of the jejunum preparations with STR or verapamil in Ca-free high-K (60 mM) solution containing EDTA. STR (125, 250 and 500 mg/kg) exhibited antidiarrheal activity. STR (0.01-10 mg/mL) completely relaxed spontaneously contracting, Ach (10M) and high K (60 mM) induced contracted jejunum with an EC value of 0.66 (0.49-0.96), 0.39 (0.28-0.44) and 0.17 (0.10-0.21), similar to verapamil. Concentration-response curves of CaCl could be significantly moved to the right and down in the presence of STR (0.3, 1 mg/mL). Results suggest the presence of antidiarrheal activity and spasmolytic effects of STR, possibly mediated through Ca channel blocking activity, providing the pharmacological basis for its traditional uses in gastrointestinal disorders.
在中国，草药甘皮（豆科，ST）（国家药典委员会。2015 年）具有抗炎、抗肿瘤和抗病毒作用。然而，迄今为止，对其胃肠道作用的研究甚少。评估了 ST 根茎的甲醇提取物（STR）的胃肠道作用。该研究于 2018 年 2 月至 12 月进行。在蓖麻油诱导的腹泻小鼠中研究了 STR（125、250 和 500mg/kg；口服）的抗腹泻活性。还研究了 STR（0.01-10mg/ml）对兔空肠分离组织制剂的影响，用 Ach（10M）、K（60mM）预收缩兔空肠条，在 STR 存在下测试，分析 STR 或维拉帕米预处理空肠制剂在含有 EDTA 的高 K（60mM）溶液中无 Ca 时的可能解痉作用。STR（125、250 和 500mg/kg）表现出抗腹泻活性。STR（0.01-10mg/ml）完全松弛自发性收缩、Ach（10M）和高 K（60mM）诱导收缩的空肠，EC 值分别为 0.66（0.49-0.96）、0.39（0.28-0.44）和 0.17（0.10-0.21），与维拉帕米相似。在 STR（0.3、1mg/ml）存在下，CaCl 的浓度-反应曲线可明显向右和向下移动。结果表明 STR 具有抗腹泻活性和抗痉挛作用，可能通过钙通道阻断活性介导，为其在胃肠道疾病中的传统应用提供了药理学基础。
