Pramudya Manikya, Wahyuningsih Sri Puji Astuti
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.
Vet World. 2019 Jun;12(6):735-739. doi: 10.14202/vetworld.2019.735-739. Epub 2019 Jun 1.
For many years, people use natural products from the plant and fungal to improve immune response against microorganism. This study aimed to investigate the immunomodulatory properties of polysaccharides (PS) from in mice infected by intracellular bacteria .
Thirty-six female BALB/C mice were divided into six groups: Normal control, negative control, positive control, P1 (PS before infection), P2 (PS after infection), and P3 (PS before and after infection). PS were administrated for 10 days. was infected twice with 2 weeks gap from the first to second exposure with a dose of 10 cells. 1 week after the end of treatment, level of oxidants, innate immune responses, and adaptive immune responses were measured.
This study showed that PS administration could restore the number of leukocytes as normal but could not enhance the number of phagocytes and its activity. PS administration also showed immunosuppression activity by lowering nitric oxide levels in P2 and P3 groups (p<0.05). This result showed that PS prevent over-expression of pro-inflammatory cytokines by decreasing phagocytic activity. Contrast with innate immune response result; PS administration could significantly increase interferon-gamma level in P1, P2, and P3 groups (p<0.05). Level of antibodies was significantly increased in the P3 group (p<0.05). PS administration also showed an increased level of tumor necrosis factor-α, but the difference was not significant (p>0.05).
PS enhance adaptive immunity due to the capability of that able to survive and replicate in phagocytes. Thus, PS from could be potentially be used as a natural immunomodulator against intracellular bacteria.
多年来，人们一直使用植物和真菌中的天然产物来改善针对微生物的免疫反应。本研究旨在探讨[具体植物名称]多糖（PS）对感染细胞内细菌的小鼠的免疫调节特性。
将36只雌性BALB/C小鼠分为六组：正常对照组、阴性对照组、阳性对照组、P1组（感染前给予PS）、P2组（感染后给予PS）和P3组（感染前后均给予PS）。连续10天给予PS。[具体细菌名称]分两次感染，第一次和第二次感染间隔2周，剂量为10个细胞。治疗结束后1周，检测氧化剂水平、固有免疫反应和适应性免疫反应。
本研究表明，给予PS可使白细胞数量恢复正常，但不能增加吞噬细胞数量及其活性。给予PS还通过降低P2组和P3组的一氧化氮水平显示出免疫抑制活性（p<0.05）。这一结果表明，PS通过降低吞噬活性来防止促炎细胞因子的过度表达。与固有免疫反应结果相反；给予PS可使P1、P2和P3组的干扰素-γ水平显著升高（p<0.05）。P3组抗体水平显著升高（p<0.05）。给予PS也使肿瘤坏死因子-α水平升高，但差异不显著（p>0.05）。
由于[具体细菌名称]能够在吞噬细胞内存活和复制，PS增强了适应性免疫。因此，[具体植物名称]的PS有可能被用作针对细胞内细菌的天然免疫调节剂。
