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评估一种利用半纤维素玉米芯液体制备用于生产表面活性素的培养基的新策略。

Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor.

作者信息

Prado Aline Alves Oliveira Santos, Santos Brenda Lohanny Passos, Vieira Isabela Maria Monteiro, Ramos Larissa Castor, de Souza Roberto Rodrigues, Silva Daniel Pereira, Ruzene Denise Santos

机构信息

Northeastern Biotechnology Network, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil.

Coordination of Food Technical Course, Federal Institute of Sergipe, 49055-260, Aracaju, SE, Brazil.

出版信息

Biotechnol Rep (Amst). 2019 Jul 30;24:e00364. doi: 10.1016/j.btre.2019.e00364. eCollection 2019 Dec.

Abstract

The biosurfactant production is characterized by high costs with substrates, which does not make them sufficiently competitive against synthetic surfactants. The insertion of alternative sources of low cost, especially agro-industrial residue, is an excellent alternative to make this competitiveness viable. An alkaline pretreatment was used to extract the hemicellulose from corncob in order to enhance its C5 fraction, common to vegetable biomasses. The hemicellulosic corncob liquor was used with glucose and mineral salt solution as carbon and nutrients sources in a fermentation process for the growth of . It was performed a 2 full factorial design to determine the best conditions for the surfactin production in relation to the following response variables: surface tension reduction rate (STRR) and emulsification index (EI), from which were obtained two optimized bioproducts under specific conditions. The optimized biosurfactants found to be effected presenting a critical micelle concentration of 100 mg.L and a maximum bioremediation potential of 85.18%, as well as maximum values of 57.38% and 65.30% for STRR and EI variables, respectively. Overall results pointed for a successful commercial application for the surfactin produced.

摘要

生物表面活性剂的生产具有底物成本高的特点,这使其在与合成表面活性剂的竞争中缺乏足够的竞争力。引入低成本的替代来源,尤其是农业工业残渣,是实现这种竞争力的绝佳选择。采用碱性预处理从玉米芯中提取半纤维素,以提高其C5组分,这是植物生物质中常见的成分。半纤维素玉米芯液与葡萄糖和矿物盐溶液一起用作发酵过程中的碳源和营养源,用于[具体微生物名称未给出]的生长。进行了二因素全因子设计,以确定与以下响应变量相关的表面活性素生产的最佳条件:表面张力降低率(STRR)和乳化指数(EI),在特定条件下从中获得了两种优化的生物产品。发现优化后的生物表面活性剂具有100 mg.L的临界胶束浓度和85.18%的最大生物修复潜力,STRR和EI变量的最大值分别为57.38%和65.30%。总体结果表明所生产的表面活性素具有成功的商业应用前景。

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