Suppr超能文献

来自高通量测序的18S rRNA基因扩增子文库的分类注释和多样性数据。

Data on taxonomic annotation and diversity of 18S rRNA gene amplicon libraries derived from high throughput sequencing.

作者信息

Kataoka Takafumi, Kondo Ryuji

机构信息

Department of Marine Science and Technology, Fukui Prefectural University, Obama, Fukui, 917-0003, Japan.

出版信息

Data Brief. 2019 Jul 2;25:104213. doi: 10.1016/j.dib.2019.104213. eCollection 2019 Aug.

Abstract

This Data in Brief article is a supporting information for the research article entitled "Protistan community composition in anoxic sediments from three salinity-disparate Japanese lakes" by Kataoka and Kondo (2019) [1]. Summary of 18S rRNA gene sequences originated from anoxic sediment of three lakes in two seasons using high throughput sequencing techniques (MiSeq, Illumina) was shown in this data article. Supergroup-level taxonomy was compared between the SILVA search for SILVA database and BLASTn search for the PR2 database. Alpha diversity was calculated in each sample, and beta-diversity was calculated among the six amplicon libraries. Partial sequence length between the primer set of 574*f and 1132R Hugerth et al., 2015 was compared between the forward read and the combined read.

摘要

这篇《数据简报》文章是Kataoka和Kondo(2019年)[1]发表的题为《来自三个盐度不同的日本湖泊缺氧沉积物中的原生生物群落组成》的研究文章的支持信息。本文展示了使用高通量测序技术(MiSeq,Illumina)对两个季节中三个湖泊缺氧沉积物的18S rRNA基因序列的总结。对SILVA数据库的SILVA搜索和PR2数据库的BLASTn搜索之间的超群水平分类法进行了比较。计算了每个样本的α多样性,并计算了六个扩增子文库之间的β多样性。比较了正向读取和合并读取之间574*f和1132R Hugerth等人(2015年)引物组之间的部分序列长度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee6f/6699457/8f8e3a40a31a/gr1.jpg

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验