Kataoka Takafumi, Kondo Ryuji
Department of Marine Science and Technology, Fukui Prefectural University, Obama, Fukui, 917-0003, Japan.
Data Brief. 2019 Jul 2;25:104213. doi: 10.1016/j.dib.2019.104213. eCollection 2019 Aug.
This Data in Brief article is a supporting information for the research article entitled "Protistan community composition in anoxic sediments from three salinity-disparate Japanese lakes" by Kataoka and Kondo (2019) [1]. Summary of 18S rRNA gene sequences originated from anoxic sediment of three lakes in two seasons using high throughput sequencing techniques (MiSeq, Illumina) was shown in this data article. Supergroup-level taxonomy was compared between the SILVA search for SILVA database and BLASTn search for the PR2 database. Alpha diversity was calculated in each sample, and beta-diversity was calculated among the six amplicon libraries. Partial sequence length between the primer set of 574*f and 1132R Hugerth et al., 2015 was compared between the forward read and the combined read.
这篇《数据简报》文章是Kataoka和Kondo(2019年)[1]发表的题为《来自三个盐度不同的日本湖泊缺氧沉积物中的原生生物群落组成》的研究文章的支持信息。本文展示了使用高通量测序技术(MiSeq,Illumina)对两个季节中三个湖泊缺氧沉积物的18S rRNA基因序列的总结。对SILVA数据库的SILVA搜索和PR2数据库的BLASTn搜索之间的超群水平分类法进行了比较。计算了每个样本的α多样性,并计算了六个扩增子文库之间的β多样性。比较了正向读取和合并读取之间574*f和1132R Hugerth等人(2015年)引物组之间的部分序列长度。