Timens W, Boes A, Rozeboom-Uiterwijk T, Poppema S
Department of Pathology, University of Groningen, The Netherlands.
Virchows Arch A Pathol Anat Histopathol. 1988;413(6):563-71. doi: 10.1007/BF00750398.
Spleen, thymus and lymph node of human fetuses from the 12th to the 38rd week (spleen from 9 weeks) were investigated in an immunohistological study on B5-fixed paraffin embedded tissues, employing a panel of recently developed monoclonal antibodies, reactive with antigens resistant against fixation and paraffin embedment. The monoclonal antibodies included were MT1, MT2, MB1, MB2, MB3, LN1, LN2, LN3, LeuM1, Leu7, VIE-G4, together with polyclonal antibodies reactive with immunoglobulin heavy and light chains, and with lysozyme and S100-protein. The preservation of morphological detail together with immunoperoxidase staining of cellular subsets, allowed an accurate determination of the ontogenic development of the different cell types in situ, in relation to their micro-environment. The use of paraffin tissue reactive (monoclonal) antibodies gives an extra dimension to the study of fetal lymphoid tissues. This is of particular advantage in studies on very fragile tissues as in early embryonal and fetal ontogeny.
在一项针对B5固定石蜡包埋组织的免疫组织学研究中，我们使用了一组最近开发的单克隆抗体，这些抗体可与抗固定和石蜡包埋的抗原发生反应，对妊娠12至38周（脾脏从9周起）的人类胎儿的脾脏、胸腺和淋巴结进行了研究。所使用的单克隆抗体包括MT1、MT2、MB1、MB2、MB3、LN1、LN2、LN3、LeuM1、Leu7、VIE-G4，同时还使用了与免疫球蛋白重链和轻链、溶菌酶及S100蛋白发生反应的多克隆抗体。形态细节的保留以及细胞亚群的免疫过氧化物酶染色，使得能够准确确定不同细胞类型在原位与其微环境相关的个体发生发育情况。使用石蜡组织反应性（单克隆）抗体为胎儿淋巴组织的研究增添了新的维度。这在研究如早期胚胎和胎儿个体发育中非常脆弱的组织时具有特别的优势。
