Warnke R A, Gatter K C, Falini B, Hildreth P, Woolston R E, Pulford K, Cordell J L, Cohen B, De Wolf-Peeters C, Mason D Y
N Engl J Med. 1983 Nov 24;309(21):1275-81. doi: 10.1056/NEJM198311243092102.
Two monoclonal antibodies have been produced that react with antigens present on human white cells. These reagents differ from other monoclonal antibodies of similar specificity in that the antigens they recognize are resistant to conventional tissue-fixation and embedding procedures. These reagents can therefore be used in immunocytochemical staining of paraffin-embedded tissue sections. We assessed the practical usefulness of this technique in the histopathological diagnosis of human lymphoid neoplasms by staining a wide range of routine surgical biopsy specimens of normal and neoplastic tissue (gathered from five institutions), using an indirect immunoperoxidase technique. In all 40 cases of non-Hodgkin's lymphoma, positive labeling of neoplastic cells was obtained with one or both antibodies. In contrast, no staining of neoplastic cells was observed in 60 samples of nonlymphoid neoplasms. We conclude that many of the difficulties encountered by histopathologists in distinguishing between lymphoid and nonlymphoid neoplasms may be overcome by immunohistologic labeling with monoclonal antibodies such as the ones we have studied.
已经制备出两种与人类白细胞上存在的抗原发生反应的单克隆抗体。这些试剂与其他具有相似特异性的单克隆抗体不同，在于它们识别的抗原对传统的组织固定和包埋程序具有抗性。因此，这些试剂可用于石蜡包埋组织切片的免疫细胞化学染色。我们通过使用间接免疫过氧化物酶技术对来自五个机构的各种正常和肿瘤组织的常规手术活检标本进行染色，评估了该技术在人类淋巴肿瘤组织病理学诊断中的实际实用性。在所有40例非霍奇金淋巴瘤病例中，使用一种或两种抗体均获得了肿瘤细胞的阳性标记。相比之下，在60例非淋巴肿瘤样本中未观察到肿瘤细胞染色。我们得出结论，组织病理学家在区分淋巴肿瘤和非淋巴肿瘤时遇到的许多困难可以通过使用我们所研究的这类单克隆抗体进行免疫组织学标记来克服。
