Department of Microbiology, Abbottabad University of Science & Technology, Abbottabad, 22010, Pakistan.
Department of Microbiology, Hazara University, Mansehra, 21300, Pakistan.
Environ Monit Assess. 2019 Aug 22;191(9):585. doi: 10.1007/s10661-019-7769-5.
Remediation of heavy metals, other than microbial bioleaching method, is expensive and unsuitable for large contaminated areas. The current study was aimed to isolate, identify, and test the potential of indigenous fungal strains for heavy metal removal from contaminated soil. A total of three metallotolerant fungal strains, i.e., Aspergillus niger (M1DGR), Aspergillus fumigatus (M3Ai), and Penicillium rubens (M2Aii), were isolated and identified by phenotyping and genotyping from heavy metal-contaminated soil of Hattar Industrial Estate, Pakistan. A. niger was found to be the most successful strain for the removal of heavy metals from the contaminated soil with maximum bioaccumulation efficiency of 98% (Cd) and 43% (Cr). In contrast, A. fumigatus showed comparatively low but still considerable bioleaching potential, i.e., 79% and 69% for Cd and Cr removal, respectively. Maximum metal uptake efficiency, i.e., 0.580 mg g and 0.152 mg g by A. niger strain was noticed for Cd and Cr with Czapek yeast extract (CYE) and Sabouraud dextrose broth (SDB) media, respectively. A. fumigatus (M3Ai) exhibited the maximum bioleaching capacity (0.40 mg g) for Cr with CYE medium. The results reveal that A. niger M1DGR and A. fumigatus M3Ai could be used to develop new strategies to remediate soil contaminated with heavy metals (Cd and Cr) through either in situ or ex situ mycoremediation.
除了微生物生物浸出法之外，其他重金属修复方法都很昂贵，不适合大面积污染区域。本研究旨在从受污染土壤中分离、鉴定和测试本土真菌菌株对重金属的去除潜力。从巴基斯坦哈塔尔工业区受重金属污染的土壤中，通过表型和基因型鉴定，共分离出 3 株耐金属真菌菌株，即黑曲霉（M1DGR）、烟曲霉（M3Ai）和红曲霉（M2Aii）。黑曲霉被发现是从污染土壤中去除重金属最成功的菌株，其最大生物积累效率分别为 98%（Cd）和 43%（Cr）。相比之下，烟曲霉表现出相对较低但仍相当可观的生物浸出潜力，分别为 Cd 和 Cr 的 79%和 69%。黑曲霉在酵母提取物查氏培养基（CYE）和沙氏葡萄糖肉汤培养基（SDB）中对 Cd 和 Cr 的最大金属吸收效率分别为 0.580 mg/g 和 0.152 mg/g。烟曲霉（M3Ai）在 CYE 培养基中对 Cr 的生物浸出能力最大（0.40 mg/g）。结果表明，黑曲霉 M1DGR 和烟曲霉 M3Ai 可用于开发新策略，通过原位或异位菌根修复来修复受重金属（Cd 和 Cr）污染的土壤。
