Daniel-González Guadalupe L, Nava-Galicia Soley B, Arroyo-Becerra Analilia, Villalobos-López Miguel Angel, Díaz-Godínez Gerardo, Bibbins-Martínez Martha D
Centro de Investigación en Biotecnología Aplicada (CIBA), Instituto Politécnico Nacional, Tlaxcala 90700, Mexico.
Research Center for Biological Sciences, Autonomous University of Tlaxcala, Tlaxcala 90000, Mexico.
J Fungi (Basel). 2025 Jul 31;11(8):572. doi: 10.3390/jof11080572.
Synthetic dyes are highly recalcitrant and are discharged in large volumes in industrial wastewater, which represents a serious environmental pollution problem. Biological methods for dye degradation are a potentially effective option for these synthetic products. In this study, a strain of was used to evaluate the decolorization of the Remazol Brilliant Blue R (RBBR) dye added to the culture medium in the exponential growth phase of the fungus. The dye removal capacity of live and inactivated pellets by biosorption, as well as the enzymatic degradation of the dye using a cell-free culture broth considered an extracellular extract (EE), were also evaluated. The activity of laccase and dye-decolorizing peroxidase was determined in both the EE and the intrapellet extract (IPE); their values increased in the presence of dye in the culture medium. A decolorization of 98.5% and 98.0% was obtained in the culture broth and by the EE, respectively; biosorption of the dye by the inactivated pellets was 17 mg/g. The results suggest that the decolorization of the dye is primarily enzymatic, although there are also bioadsorption and bioaccumulation of the dye, which is then enzymatically degraded, and could be used as a carbon source.
合成染料具有高度难降解性，大量排放于工业废水中，这是一个严重的环境污染问题。生物法降解染料是处理这些合成产品的一种潜在有效选择。在本研究中，使用了一株[具体菌株未给出]来评估在真菌指数生长期添加到培养基中的雷马素亮蓝R（RBBR）染料的脱色情况。还评估了活的和灭活的菌球通过生物吸附对染料的去除能力，以及使用被视为细胞外提取物（EE）的无细胞培养液对染料的酶促降解。在EE和菌球内提取物（IPE）中测定了漆酶和染料脱色过氧化物酶的活性；在培养基中存在染料的情况下，它们的值会增加。在培养液和EE中分别实现了98.5%和98.0%的脱色；灭活菌球对染料的生物吸附量为17 mg/g。结果表明，染料的脱色主要是酶促作用，尽管也存在染料的生物吸附和生物积累，随后染料被酶促降解，并且可以用作碳源。
