College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Ecotoxicol Environ Saf. 2019 Nov 15;183:109575. doi: 10.1016/j.ecoenv.2019.109575. Epub 2019 Aug 20.
Tibial Dyschondroplasia (TD), a metabolic disease of fast growing poultry birds that effects the growth of bone and cartilage, is characterized by anorexia, mental depression and lameness. Wnt/β-catenin pathway can mediate the occurrence of TD, and previous study showed the therapeutic effect of TanshinoneⅡA to TD Broilers. However there is no report about the effect of TanshinoneⅡA treating TD broiler chicken through wnt/β-catenin pathway. The objective of this study was to explore the potential mechanism of how Tanshinone II A treats TD. Hematoxylin and eosin staining was used to study histologic pathology of growth plates. Key gene expressions were tested by western blot and reverse transcription quantitative real-time PCR. Results compared with control groups, showed the TD broilers' growth plate performed significantly better by treating with TanshinoneⅡA. After chickens treated by TanshinoneⅡA, the gene and protein expression of WNT5α and BMP-2 were increased (P < 0.05), but the β-catenin were decreased (P < 0.05), which are all key genes expressed in wnt/β-catenin pathway. Therefore, TanshinoneⅡA can potentially treat TD by affecting the expression of genes in wnt/β-catenin pathway and it has availability to use as treatment for TD broilers.
胫骨软骨发育不良（TD）是一种影响骨骼和软骨生长的快速生长家禽鸟类的代谢疾病，其特征为食欲不振、精神抑郁和跛行。Wnt/β-catenin 通路可以介导 TD 的发生，先前的研究表明丹参酮ⅡA 对 TD 肉仔鸡具有治疗作用。然而，关于丹参酮ⅡA 通过 wnt/β-catenin 通路治疗 TD 肉仔鸡的效果尚无报道。本研究旨在探讨丹参酮ⅡA 治疗 TD 的潜在机制。通过苏木精和伊红染色研究生长板的组织病理学。通过 Western blot 和反转录定量实时 PCR 测试关键基因的表达。结果与对照组相比，丹参酮ⅡA 处理的 TD 肉仔鸡的生长板表现出明显改善。用丹参酮ⅡA 处理后，WNT5α 和 BMP-2 的基因和蛋白表达增加（P<0.05），而β-catenin 减少（P<0.05），这都是 wnt/β-catenin 通路中表达的关键基因。因此，丹参酮ⅡA 可能通过影响 wnt/β-catenin 通路中基因的表达来潜在地治疗 TD，并且它具有作为 TD 肉仔鸡治疗剂的应用潜力。
