State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/ Guangdong litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/ Guangdong litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou, China.
Plant Physiol Biochem. 2019 Sep;142:490-499. doi: 10.1016/j.plaphy.2019.08.007. Epub 2019 Aug 10.
ICE1 (inducer of CBF expression 1) encodes a typical MYC-like basic helix-loop- helix (bHLH) transcription factor that acts as a pivotal component in the cold signalling pathway. In this study, DlICE1, a novel ICE1-like gene, was isolated from the southern subtropical fruit tree longan (Dimocarpus longan Lour.). DlICE1 encodes a nuclear protein with a highly conserved bHLH domain. DlICE1 expression was slightly upregulated under cold stress. Overexpression of DlICE1 in Arabidopsis conferred enhanced cold tolerance via increased proline content, decreased ion leakage, and reduced malondialdehyde (MDA) and reactive oxygen species (ROS) accumulation. Expression of the ICE1-CBF cold signalling pathway genes, including AtCBF1/2/3 and cold-responsive genes (AtRD29A, AtCOR15A, AtCOR47 and AtKIN1), was also significantly higher in DlICE1-overexpressing lines than in wild-type (WT) plants under cold stress. In conclusion, these findings indicate that DlICE1 is a member of the bHLH gene family and positively regulates cold tolerance in D. longan.
ICE1（CBF 表达诱导因子 1）编码一种典型的 MYC 样碱性螺旋-环-螺旋（bHLH）转录因子，作为冷信号通路中的关键组成部分。在这项研究中，从南方亚热带果树龙眼（Dimocarpus longan Lour.）中分离出一种新型的 ICE1 样基因 DlICE1。DlICE1 编码一种具有高度保守 bHLH 结构域的核蛋白。DlICE1 的表达在冷胁迫下略有上调。在拟南芥中过表达 DlICE1 通过增加脯氨酸含量、降低离子渗漏以及减少丙二醛（MDA）和活性氧（ROS）积累来赋予增强的耐寒性。ICE1-CBF 冷信号通路基因（包括 AtCBF1/2/3 和冷响应基因（AtRD29A、AtCOR15A、AtCOR47 和 AtKIN1）的表达在冷胁迫下也明显高于野生型（WT）植物中的 DlICE1 过表达系。总之，这些发现表明 DlICE1 是 bHLH 基因家族的成员，并在龙眼中正向调节耐寒性。
