Institute of Vegetable Research, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, China.
Institute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, China.
Sci Rep. 2024 Sep 27;14(1):22181. doi: 10.1038/s41598-024-73135-y.
Traditional substrate cultivation is now a routine practice in vegetable facility breeding. However, finding renewable substrates that can replace traditional substrates is urgent in today's production. In this study, we used the 'Pindstrup' substrate as control and two types of composite substrates made from fermented corn straw (i.e. 0-3 and 3-5 mm) to identify appropriate substrate conditions for tomato seedling growth under winter greenhouse conditions. Seedling growth potential related data and substrate water content related data were tested to carry out data-oriented support. Since the single physiological data cannot well explain the mechanism of tomato seedlings under winter greenhouse condition, transcriptomic analysis of tomato root and leaf tissues were conducted to provide theoretical basis. The physiological data of tomato seedlings and substrate showed that compared with 0-3 mm and Pindstrup substrate, tomato seedlings planted in 3-5 mm had stronger growth potential and stronger water retention, and were more suitable for planting tomato seedlings. Transcriptome analysis revealed a greater number of DEGs between the Pindstrup and the 3-5 mm. The genes in this group contribute to tomato growth as well as tomato stress response mechanisms, such as ABA-related genes, hormone-related genes and some TFs. The simulation network mechanism diagram adds evidence to the above conclusions. Overall, these results demonstrate the potential benefits of using the fermented corn straw of 3-5 mm for growing tomato seedlings and present a novel method of utilizing corn straw.
传统基质栽培现在是蔬菜设施育种的常规做法。然而，在当今的生产中，寻找可替代传统基质的可再生基质迫在眉睫。在本研究中，我们以‘Pindstrup’基质为对照，以两种发酵玉米秸秆（0-3 和 3-5 毫米）制成的复合基质，来确定适合番茄幼苗在冬季温室条件下生长的基质条件。测试了与幼苗生长潜力相关的数据和与基质含水量相关的数据，以进行数据导向的支持。由于单一的生理数据不能很好地解释番茄幼苗在冬季温室条件下的机制，我们对番茄根和叶组织进行了转录组分析，为其提供理论依据。番茄幼苗和基质的生理数据表明，与 0-3 毫米和 Pindstrup 基质相比，种植在 3-5 毫米的番茄幼苗具有更强的生长潜力和更强的保水能力，更适合种植番茄幼苗。转录组分析显示，Pindstrup 和 3-5 毫米之间存在更多的 DEGs。该组中的基因有助于番茄生长以及番茄对胁迫的反应机制，如 ABA 相关基因、激素相关基因和一些 TFs。模拟网络机制图为上述结论提供了证据。总的来说，这些结果表明，使用 3-5 毫米发酵玉米秸秆种植番茄幼苗具有潜在的好处，并为利用玉米秸秆提供了一种新方法。
