Nguyen E, Pajot-Augy E, Campion E, Prulière G
Unité de Recherche en Développement concerté I.N.S.E.R.M.-I.N.R.A. n. 310, Institut de Biologie physico-chimique, Paris.
C R Acad Sci III. 1988;307(3):93-8.
The influence of 1,2-propanediol on the G-actin/F-actin equilibrium has been studied previously. We report here its effect on the crosslinking of actin filaments by alpha-actinin. Capillary viscometry experiments indicate a marked increase in the viscosity of F-actin/alpha-actinin solutions in the presence of this solvent, with a rise of the gel point temperature. Gel electrophoresis obtained after high-speed sedimentation of these proteins showed an enhancement of the alpha-actinin/actin filaments association. Propanediol-induced perturbations of interactions between microfilaments and actin-binding proteins might help explain the variability of its cryoprotective efficiency for the preservation of various cells.
1,2 - 丙二醇对G - 肌动蛋白/F - 肌动蛋白平衡的影响此前已有研究。我们在此报告其对α - 辅肌动蛋白介导的肌动蛋白丝交联的影响。毛细管粘度测定实验表明，在这种溶剂存在下，F - 肌动蛋白/α - 辅肌动蛋白溶液的粘度显著增加，凝胶化温度升高。对这些蛋白质进行高速沉降后得到的凝胶电泳显示α - 辅肌动蛋白与肌动蛋白丝的结合增强。丙二醇引起的微丝与肌动蛋白结合蛋白之间相互作用的扰动可能有助于解释其对各种细胞保存的冷冻保护效率的差异。
