Vernole P, Tedeschi B, Caporossi D, Nicoletti B
Department of Public Health and Cellular Biology, 2nd University of Rome, Italy.
Cancer Genet Cytogenet. 1988 Nov;36(1):13-23. doi: 10.1016/0165-4608(88)90070-2.
To clarify the possible relationship between fragile site expression and cancer, we examined lymphocytes from patients affected by neuroblastoma. This neoplasia may be inherited in some cases and is often characterized by a specific chromosomal aberration: deletion of the short arm of chromosome 1. We found a higher expression of fragile sites after aphidicolin and, to a lesser extent, after methotrexate treatment in lymphocytes from neuroblastoma patients as compared with those of normal donors. The analysis of fragile site distribution pointed out the increase in the expression of fragile site 1p32 in the patients. We believe that this finding might be relevant because this fragile site is located in the same region where breakpoints and rearrangements frequently occur in neuroblastoma cells.
为了阐明脆性位点表达与癌症之间的可能关系，我们检测了神经母细胞瘤患者的淋巴细胞。这种肿瘤在某些情况下可能是遗传性的，其特征通常是特定的染色体畸变：1号染色体短臂缺失。我们发现，与正常供体的淋巴细胞相比，阿非科林处理后神经母细胞瘤患者的淋巴细胞中脆性位点的表达更高，甲氨蝶呤处理后的表达升高程度较小。对脆性位点分布的分析指出，患者中脆性位点1p32的表达增加。我们认为这一发现可能具有相关性，因为该脆性位点位于神经母细胞瘤细胞中经常出现断点和重排的同一区域。
