Nishita T, Matsushita H, Kai M
Laboratory of Veterinary Physiology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Equine Vet J. 1987 Nov;19(6):509-13. doi: 10.1111/j.2042-3306.1987.tb02660.x.
The location of carbonic anhydrase III (CA-III) in frozen sections of biopsies of Thoroughbred horse skeletal muscle was studied. Fibre types were determined by ATP-ase and succinate dehydrogenase staining. CA-III isozyme was detected using a peroxidase conjugated anti-CA-III antibody. CA-III was found to be localised in slow twitch oxidative fibres (ST), but was also present in fast twitch oxidative (FTH) fibres in small amounts. Fast twitch glycolytic (FT) fibres were stained lightly compared with control sections. The concentrations of CA-III in muscle and liver were 70 micrograms/mg protein and 4 micrograms/mg protein, respectively. CA-I and CA-II were not found in muscle extracts by the double immunodiffusion method.
对纯种马骨骼肌活检冷冻切片中碳酸酐酶III（CA-III）的定位进行了研究。通过ATP酶和琥珀酸脱氢酶染色确定纤维类型。使用过氧化物酶偶联的抗CA-III抗体检测CA-III同工酶。发现CA-III定位于慢肌氧化纤维（ST）中，但也少量存在于快肌氧化（FTH）纤维中。与对照切片相比，快肌糖酵解（FT）纤维染色较浅。肌肉和肝脏中CA-III的浓度分别为70微克/毫克蛋白质和4微克/毫克蛋白质。通过双向免疫扩散法在肌肉提取物中未发现CA-I和CA-II。
