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马及其他哺乳动物物种中碳酸酐酶III的比较免疫化学研究。

Comparative immunochemical studies of carbonic anhydrase III in horses and other mammalian species.

作者信息

Nishita T, Matsushita H

机构信息

Laboratory of Veterinary Physiology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.

出版信息

Comp Biochem Physiol B. 1988;91(1):91-6. doi: 10.1016/0305-0491(88)90118-6.

DOI:10.1016/0305-0491(88)90118-6
PMID:3143514
Abstract
  1. Carbonic anhydrase III (CA-III) from different mammalian species (horse, cow, dog, cat, rat and rabbit) has been analyzed by the immunodiffusion technique with anti-equine CA-III serum. 2. Immunodiffusion demonstrated the absence of cross-reactivity between isozyme CA-I, CA-II, and CA-III. 3. Cross-reactions were observed between the CA-III from all the species examined except the rabbit. 4. Molecular weights and isoelectric points of CA-III from different species were determined by Western blotting.
摘要
  1. 利用抗马碳酸酐酶III血清,通过免疫扩散技术对来自不同哺乳动物物种(马、牛、狗、猫、大鼠和兔子)的碳酸酐酶III(CA-III)进行了分析。2. 免疫扩散显示同工酶CA-I、CA-II和CA-III之间不存在交叉反应。3. 在所检测的所有物种(除兔子外)的CA-III之间观察到了交叉反应。4. 通过蛋白质免疫印迹法测定了不同物种CA-III的分子量和等电点。

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Comp Biochem Physiol B. 1988;91(1):91-6. doi: 10.1016/0305-0491(88)90118-6.
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