Lee Young Ju, Lee Jae-Chul, Eun Young Gyu, Lee Gi-Ja
Dept. of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul, 02447, South Korea.
Human Convergence Technology Group, Korea Institute of Industrial Technology, Ansan, 15588, South Korea.
Anal Biochem. 2019 Nov 15;585:113404. doi: 10.1016/j.ab.2019.113404. Epub 2019 Aug 21.
Nasal secretions (NS) reflect inflammatory activity of the nasal mucosa and thus can be utilized for disease diagnosis and determining treatment effects in Allergic rhinitis (AR). However, non-standardized collection of samples can affect the measured concentration of inflammatory biomarker in NS. In this study, we aimed to develop and evaluate new devices capable of standardizing the collection, storage, and preprocessing methods of NS samples. First, we chose the best swab as polyester (PE) and selected a stimulation method, twirling for 10 s at 1 Hz, to efficiently release AR biomarkers from a PE swab. Storage of sample solutions at -20 °C was optimal for the stability of biomarkers for the detection of AR. The new swab sample transfer device showed excellent concentration recovery efficiency (90-100%) for tryptase (Trp) and eosinophil cationic protein (ECP) without crosstalk between the two biomarkers. Finally, we compared the concentration of Trp in human NS samples of AR patients (n = 6) pre-processed by the new device with that by centrifuge as a standard method. As a result, the concentrations of Trp in NS were very similar in both groups. Therefore, this device can be utilized as an effective sample transfer and pre-processing device for point-of-care testing of AR.
鼻分泌物（NS）反映了鼻黏膜的炎症活动，因此可用于变应性鼻炎（AR）的疾病诊断和治疗效果判定。然而，样本采集的不标准化会影响NS中炎症生物标志物的测量浓度。在本研究中，我们旨在研发并评估能够使NS样本的采集、储存和预处理方法标准化的新装置。首先，我们选择聚酯（PE）拭子作为最佳拭子，并选择一种刺激方法，即以1Hz旋转10秒，以有效地从PE拭子中释放AR生物标志物。将样本溶液储存在-20°C对用于检测AR的生物标志物的稳定性最为理想。新型拭子样本转移装置对类胰蛋白酶（Trp）和嗜酸性粒细胞阳离子蛋白（ECP）显示出优异的浓度回收效率（90-100%），且两种生物标志物之间无串扰。最后，我们将新装置预处理的AR患者（n = 6）的人NS样本中Trp的浓度与作为标准方法的离心法预处理的样本进行了比较。结果，两组NS中Trp的浓度非常相似。因此，该装置可作为AR即时检测的有效样本转移和预处理装置。
