Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
Gen Comp Endocrinol. 2020 Jan 1;285:113249. doi: 10.1016/j.ygcen.2019.113249. Epub 2019 Aug 21.
The objective of this study was to document the expression and functional role of BMPs in the placental (caruncle; CAR, cotyledon; COT) during different stages of pregnancy in water buffalo. Samples collected from Early pregnancy 1 (EP1); Early pregnancy 2 (EP2), Mid pregnancy (MP), Late pregnancy (LP) while the third stage of oestrus cycle (NP) was taken as control. Also, the synergistic role of BMP4/BMP7 or combination on mRNA expression of vWF, PCNA, StAR, CYP11A1, 3βHSD, and BAX were studied in trophoblast cells cultured (TCC) during an early stage. The qPCR and immunoblotting studies revealed that BMP2, BMPR1A, BMPR1B, and BMPR2 mRNA level was significantly (p < 0.05) upregulated during early pregnancy in COTs while in CARs it was significantly upregulated (p < 0.05) during all the stages of pregnancy.BMP4 mRNA level was significantly upregulated (p < 0.05) during early pregnancy in COTs as well as in CARs. BMP6 expression was significantly upregulated (p < 0.05) during early and late stages of pregnancy. BMP7 mRNA level was upregulated (p < 0.05) during the late stage of pregnancy in COTs. At 100 ng/ml, the BMP4 maximally stimulated the transcripts of StAR, CYP11A1, and 3βHSD while BMP7 maximally stimulated the transcripts of 3βHSD that paralleled with P4 accretion in the media (P < 0.05). BMP4 as well as BMP7 upregulated the transcripts of PCNA, vWF, and downregulated BAX in the TCC (P < 0.05). In conclusion, BMPs are expressed in a regulated manner with stage-specific differences in the placenta and promotes the angiogenesis, proliferation, cell survivability, and steroidogenesis thereby regulating placental function in an autocrine/paracrine manner in water buffalo.
本研究旨在记录 BMP 在水牛妊娠不同阶段胎盘（肉阜；CAR，绒毛叶；COT）中的表达和功能作用。样本取自早期妊娠 1 期（EP1）；早期妊娠 2 期（EP2），中期妊娠（MP），晚期妊娠（LP），而发情周期的第三阶段（NP）作为对照。此外，还研究了 BMP4/BMP7 或组合在早期培养的滋养层细胞（TCC）中对 vWF、PCNA、StAR、CYP11A1、3βHSD 和 BAX mRNA 表达的协同作用。qPCR 和免疫印迹研究表明，BMP2、BMPR1A、BMPR1B 和 BMPR2 mRNA 水平在 COT 中妊娠早期显著上调（p<0.05），而在 CAR 中在整个妊娠阶段均显著上调（p<0.05）。BMP4 mRNA 水平在 COT 和 CAR 中妊娠早期均显著上调（p<0.05）。BMP6 表达在妊娠早期和晚期均显著上调（p<0.05）。BMP7 mRNA 水平在 COT 妊娠晚期上调（p<0.05）。在 100ng/ml 时，BMP4 最大程度地刺激了 StAR、CYP11A1 和 3βHSD 的转录，而 BMP7 最大程度地刺激了 3βHSD 的转录，与培养基中 P4 的积累相平行（p<0.05）。BMP4 和 BMP7 上调了 TCC 中的 PCNA、vWF 的转录，下调了 BAX（p<0.05）。总之，BMP 以调节方式表达，在胎盘中有阶段特异性差异，并以自分泌/旁分泌方式促进血管生成、增殖、细胞存活和类固醇生成，从而调节胎盘功能。
