Chemistry Department, Faculty of Sciences and Arts, University of Jeddah, Khulais, P.O. Box 355, Khulais 21921, Saudi Arabia; Chemistry Department, Faculty of Applied Science, Taiz University, Taiz, Yemen.
Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, Jeddah 21589, Saudi Arabia; Dyeing, Printing and Textile Auxiliaries Department, Textile Research Division, National Research Center, Dokki, 71516, Cairo, Egypt.
Int J Biol Macromol. 2019 Nov 1;140:949-958. doi: 10.1016/j.ijbiomac.2019.08.179. Epub 2019 Aug 21.
In this study, hydrazine treated acrylic fabrics (polyacrylonitrile, PAN) activated with cyanuric chloride was developed as supporting material for horseradish peroxidase (HRP) immobilization. The immobilization of HRP onto the modified supporting material was achieved after being end-over-end incubated for 12 h. Field emission scanning electron microscopy and Fourier-transform infrared spectroscopy techniques were used to confirm the successful immobilization. Reusability experiment was performed to estimate the ability of the immobilized HRP to recover the reaction medium, in which it was observed to retain 78% of its original activity after 10 cycles. Relative to the soluble HRP, the optimum pH and temperature for the immobilized HRP were shifted to 7-7.5 and 50 °C, respectively. The kinetic parameters of guaiacol and HO for the immobilized HRP were determined to be Km/Vmax = 57.61, 11.35 and Kcat/Km = 1.87, 1.86, respectively, while the values for the free form were Km/Vmax = 41.49, 6.23 and Kcat/Km = 1.87, 1.86, respectively. Compared to the soluble form, the immobilized HRP exhibited higher resistance toward metal ions and some organic solvents. For an application perspective. The immobilization of HRP using this procedure has the potential to be used for industrial application and wastewater treatment.
在这项研究中，制备了一种肼处理的丙烯腈纤维（聚丙烯腈，PAN），并用三聚氰胺氯活化作为辣根过氧化物酶（HRP）固定化的支撑材料。HRP 通过端到端孵育 12 小时后固定在改性支撑材料上。采用场发射扫描电子显微镜和傅里叶变换红外光谱技术确认了固定化的成功。进行了可重复使用实验以估计固定化 HRP 从反应介质中回收的能力，在 10 个循环后，它保留了 78%的原始活性。与可溶性 HRP 相比，固定化 HRP 的最佳 pH 和温度分别转移到 7-7.5 和 50°C。确定了固定化 HRP 对愈创木酚和 HO 的动力学参数为 Km/Vmax = 57.61、11.35 和 Kcat/Km = 1.87、1.86，而游离形式的 Km/Vmax = 41.49、6.23 和 Kcat/Km = 1.87、1.86。与游离形式相比，固定化 HRP 对金属离子和一些有机溶剂具有更高的抗性。从应用角度来看，使用该方法固定 HRP 有可能用于工业应用和废水处理。
