Laboratory of Animal Health Ⅱ, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan.
Laboratory of Animal Health Ⅱ, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan.
Virology. 2019 Nov;537:45-52. doi: 10.1016/j.virol.2019.08.015. Epub 2019 Aug 17.
It is important to establish the molecular basis of the high transmissibility of bovine leukemia virus (BLV) to develop new methods of preventing viral transmission. Hence, the aim of this study was to determine whether some strains had transmission advantages. First, we determined the whole BLV genome sequences of all 34 BLV-infected cows from one farm. Phylogenetic analysis divided strains into 26 major and 8 minor strains. The major strains dominantly spread independent of host factor, bovine leucocyte antigen. Further analysis, with molecular clones, associated transmissibility with viral productivity in vitro. In addition, the two groups could be classified by group-specific mutations. The reverse genetic approach demonstrated that a spontaneous mutation at nucleotide 175 of the BLV genome, which is located in the viral promoter region, could alter viral productivity by changing viral transactivation, suggesting that BLV transmissibility is affected by a spontaneous mutation associated with viral productivity.
确定牛白血病病毒 (BLV) 高传染性的分子基础对于开发新的病毒传播预防方法非常重要。因此，本研究旨在确定某些毒株是否具有传播优势。首先，我们确定了来自一个农场的 34 头 BLV 感染奶牛的整个 BLV 基因组序列。系统进化分析将毒株分为 26 个主要株系和 8 个次要株系。主要株系主要在不受宿主因素（牛白细胞抗原）影响的情况下传播。进一步的分析表明，分子克隆与体外病毒生产力相关联。此外，这两组可以通过株特异性突变来分类。反向遗传方法表明，BLV 基因组中核苷酸 175 的自发突变（位于病毒启动子区域）可能通过改变病毒的反式激活来改变病毒的生产力，这表明 BLV 的传染性受与病毒生产力相关的自发突变影响。
