Department of Virology, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey.
Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Virus Genes. 2024 Apr;60(2):173-185. doi: 10.1007/s11262-024-02058-7. Epub 2024 Feb 14.
Bovine leukemia virus is a retrovirus that causes enzootic bovine leukosis and is associated with global economic losses in the livestock industry. The aim of this study was to investigate the genotype determination of BLVs from cattle housed in 6 different farms in Türkiye and the characterization of their LTR and pX (tax, rex, R3, and G4 gene) regions. For this purpose, blood samples from 48 cattle infected with BLV were used. The phylogenetic analysis based on the env gene sequences revealed that all BLVs were clustered in genotype 1 (G1), and the sequences of the LTR (n = 48) and the pX region (n = 33) of BLVs were obtained. Also, analysis of these nucleic acid and amino acid sequences allowed assessments similar to those reported in earlier studies to be relevant to transactivation and pathogenesis. This study reports the molecular analysis of the LTR and pX region of BLVs in Türkiye for the first time.
牛白血病病毒是一种逆转录病毒，可引起牛地方性白血病，给全球畜牧业带来了巨大的经济损失。本研究旨在调查来自土耳其 6 个不同农场的牛群中 BLV 的基因型确定，以及其 LTR 和 pX（tax、rex、R3 和 G4 基因）区域的特征。为此，使用了 48 头感染 BLV 的牛的血液样本。基于 env 基因序列的系统发育分析表明，所有 BLV 均聚类在基因型 1（G1）中，获得了 BLV 的 LTR（n=48）和 pX 区（n=33）的序列。此外，对这些核酸和氨基酸序列的分析允许对与反式激活和发病机制相关的早期研究中报告的评估进行评估。本研究首次报道了土耳其 BLV 的 LTR 和 pX 区的分子分析。
