Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.
Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand; Department of Community Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Infect Genet Evol. 2019 Nov;75:104013. doi: 10.1016/j.meegid.2019.104013. Epub 2019 Aug 22.
Bufavirus (BuV), a recent discovery virus of the family Parvoviridae, has been suggested to be a potential causative agent of diarrhea in humans. The prevalence of BuV has been demonstrated mostly in fecal specimens of diarrheic patients. Little is known about the presence of BuV in the environmental water. The aim of the present study was to conduct a surveillance for BuV in the environmental water in Thailand. A total of 125 water samples were collected during November 2016 and July 2018 from six different areas in Chiang Mai city, Thailand. Water samples were concentrated and extracted to obtain BuV genomic DNA. The BuV was screened by amplification of NS1 region using nested-PCR. The detected BuV was further characterized by amplification of VP2 region. The NS1 and VP2 genes were sequenced and analyzed phylogenetically. The BuV strain (CMW88/18) detected in this study belonged to BuV1 with the prevalence of 0.8%. The CMW88/18 strain was most closely related to human BuV1 strains reported previously worldwide suggesting contamination of BuV in the environmental water could be a potential source of infection in human.
布法罗病毒（BuV）是细小病毒科的一种新发现的病毒，被认为是人类腹泻的潜在病原体。BuV 的流行主要在腹泻患者的粪便标本中得到证实。关于 BuV 在环境水中的存在知之甚少。本研究旨在对泰国环境水中的 BuV 进行监测。2016 年 11 月至 2018 年 7 月，从泰国清迈市的六个不同地区采集了 125 份水样。水样经浓缩和提取后获得 BuV 基因组 DNA。采用巢式 PCR 扩增 NS1 区对 BuV 进行筛查。对检测到的 BuV 进一步通过扩增 VP2 区进行特征分析。对 NS1 和 VP2 基因进行测序和系统进化分析。本研究中检测到的 BuV 株（CMW88/18）属于 BuV1，流行率为 0.8%。CMW88/18 株与先前在全球范围内报道的人类 BuV1 株最为密切相关，这表明环境水中的 BuV 污染可能是人类感染的潜在来源。
