Simo-Fouda Francis, Thirion Laurence, Nougairède Antoine, Luciani Léa, Driouich Jean-Sélim, Petit Paul Rémi, Delaunay Pascal, Charrel Remi N
Unité des Virus Émergents (UVE), Aix-Marseille Université, IRD 190-Inserm 1207, 13005 Marseille, France.
Inserm U1065 Centre Méditerranéen de Médecine Moléculaire, Département de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Nice (CHU Nice), 06202 Nice, France.
Pathogens. 2021 Sep 7;10(9):1151. doi: 10.3390/pathogens10091151.
Bufavirus (BuV) and human parvovirus 4 (PARV4) belong to the family. We assessed BuV and PARV4 DNA presence by real-time PCR analysis in stool, blood and respiratory samples collected in patients from Marseille and Nice, two large cities in the South-East of France. Bu-V DNA was detected in diarrheic stool samples from 92 patients (3.6% of 2583 patients), particularly men and adults, and patients from the nephrology and the infectious disease departments. Among the patients with a BuV-positive stool sample and for whom at least one blood sample was available ( = 30 patients), BuV DNA was detected also in 3 blood samples. In contrast, BuV DNA was not detected in any of the respiratory samples from 23 patients with BuV-positive stool. BuV detection rate was comparable in stool samples from patients with and without diarrhea. We did not detect PARV4 DNA in any of the stool specimens ( = 2583 patients). Our results suggest that PARV4 fecal-oral transmission is rare or non-existent in the South-East of France while BuV circulates with a relatively high rate in this area.
布法病毒（BuV）和人类细小病毒4型（PARV4）属于该病毒家族。我们通过实时聚合酶链反应（PCR）分析，对法国东南部两个大城市马赛和尼斯的患者所采集的粪便、血液和呼吸道样本中的BuV和PARV4 DNA存在情况进行了评估。在92名患者（2583名患者中的3.6%）的腹泻粪便样本中检测到了布法病毒DNA，这些患者尤其以男性、成年人以及肾病科和感染科患者为主。在粪便样本检测出布法病毒阳性且至少有一份血液样本的患者中（n = 30名患者），在3份血液样本中也检测到了布法病毒DNA。相比之下，在23名粪便样本布法病毒阳性患者的任何呼吸道样本中均未检测到布法病毒DNA。腹泻患者和未腹泻患者的粪便样本中布法病毒的检出率相当。在任何粪便标本中（n = 2583名患者）均未检测到PARV4 DNA。我们的结果表明，在法国东南部，PARV4的粪-口传播很少见或不存在，而布法病毒在该地区以相对较高的频率传播。
