The State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China; Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
The State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China; Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China; Key Laboratory of Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; The Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, 211100, China.
Curr Opin Cell Biol. 2019 Dec;61:101-109. doi: 10.1016/j.ceb.2019.07.004. Epub 2019 Aug 22.
Unravelling stem cell fate plasticity is significant for understanding pathophysiology and exploring new therapeutic targets. The Cre-loxP genetic system is commonly used for cell lineage tracing. However, this conventional approach has limitations that have led to controversies in multiple fields. In recent years, there has been growing interest in a dual genetic approach based on Cre-loxP and another orthogonal recombinase. The dual genetic approach permits cell tracking at a significantly higher resolution and enables a more precise method for gene manipulation and cell fate control. Here, we review the recent progress in dual genetic approaches and highlight emerging work to demonstrate how the application of these approaches advances our understanding of stem cell fate plasticity for tissue repair and regeneration.
解析干细胞命运可塑性对于理解病理生理学和探索新的治疗靶点具有重要意义。Cre-loxP 遗传系统常用于细胞谱系追踪。然而，这种传统方法存在局限性，导致多个领域存在争议。近年来，基于 Cre-loxP 和另一种正交重组酶的双重遗传方法引起了越来越多的关注。这种双重遗传方法可以以更高的分辨率进行细胞跟踪，并为基因操作和细胞命运控制提供更精确的方法。在这里，我们综述了双重遗传方法的最新进展，并强调了新兴的工作，以展示这些方法的应用如何促进我们对组织修复和再生中干细胞命运可塑性的理解。
