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转谷氨酰胺酶从多孔磁性纳米颗粒中的分泌及在其上的固定化

The Secretion of Transglutaminase From and Immobilization on Porous Magnetic Nanoparticles.

作者信息

Ma Tiange, Lu Jiaojiao, Zhu Jing, Li Xingjiang, Gu Hongwei, Montalbán-López Manuel, Wu Xuefeng, Luo Shuizhong, Zhao Yanyan, Jiang Shaotong, Zheng Zhi, Mu Dongdong

机构信息

School of Food and Biological Engineering, Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei, China.

State Key Laboratory of Tea Plant Biology and Utilization, School of Science, Anhui Agricultural University, Hefei, China.

出版信息

Front Microbiol. 2019 Aug 6;10:1675. doi: 10.3389/fmicb.2019.01675. eCollection 2019.

DOI:10.3389/fmicb.2019.01675
PMID:31447792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6691175/
Abstract

Microbial transglutaminase (MTG) from is an important enzyme widely applied in food processing for the improvement of protein properties by catalyzing the cross-linking of proteins. In this work we aimed at improving the production and enabling an easy and efficient purification process from culture supernatants. Thus, recombinant vectors, with either a constitutive promoter (P ) or an inducible promoter (P ), controlling the expression of the MTG gene fused to the signal peptide of Usp45 (SP ) were constructed and then expressed in . After purification, 43.5 ± 0.4 mg/L mature MTG-6His was obtained. It displayed 27.6 ± 0.5 U/mg enzymatic activity cross-linking soy protein isolate effectively. The purified mature MTG was immobilized with magnetic porous FeO nanoparticles, which improved its activity up to 29.1 ± 0.4 U/mg. The immobilized MTG maintained 67.2% of the initial activity after being recycled for 10 times. The high production and secretion of functional MTG from and the magnetic immobilized MTG-6His onto FeO nanoparticles reported in this study would have potential industrial applications.

摘要

来自[具体来源未给出]的微生物转谷氨酰胺酶（MTG）是一种重要的酶，通过催化蛋白质交联来改善蛋白质特性，在食品加工中广泛应用。在本研究中，我们旨在提高产量，并实现从培养上清液中进行简便高效的纯化过程。因此，构建了带有组成型启动子（P[具体启动子未给出]）或诱导型启动子（P[具体启动子未给出]）的重组载体，用于控制与Usp45信号肽（SP[具体信号肽未给出]）融合的MTG基因的表达，然后在[具体宿主未给出]中表达。纯化后，获得了43.5±0.4 mg/L的成熟MTG-6His。它表现出27.6±0.5 U/mg的酶活性，能有效地交联大豆分离蛋白。将纯化的成熟MTG用磁性多孔FeO纳米颗粒固定化，其活性提高到29.1±0.4 U/mg。固定化的MTG在循环使用10次后仍保持初始活性的67.2%。本研究报道的从[具体来源未给出]中高产分泌功能性MTG以及将MTG-6His磁性固定到FeO纳米颗粒上具有潜在的工业应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/8099df5cbb28/fmicb-10-01675-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/70c83ab9b4f6/fmicb-10-01675-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/7e35ee474a4a/fmicb-10-01675-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/04ef249f0369/fmicb-10-01675-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/e926551a9dd4/fmicb-10-01675-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/82476a039c28/fmicb-10-01675-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/d8f07addc3a1/fmicb-10-01675-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/04daeac1cc3e/fmicb-10-01675-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/765b874ea5f0/fmicb-10-01675-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/ef585b392aa0/fmicb-10-01675-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/8099df5cbb28/fmicb-10-01675-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/70c83ab9b4f6/fmicb-10-01675-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/7e35ee474a4a/fmicb-10-01675-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/04ef249f0369/fmicb-10-01675-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/e926551a9dd4/fmicb-10-01675-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/82476a039c28/fmicb-10-01675-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/d8f07addc3a1/fmicb-10-01675-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/04daeac1cc3e/fmicb-10-01675-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/765b874ea5f0/fmicb-10-01675-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/ef585b392aa0/fmicb-10-01675-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af71/6691175/8099df5cbb28/fmicb-10-01675-g010.jpg

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