Khashimova P R, Kondrat'eva L A, Tropina T D
Zh Mikrobiol Epidemiol Immunobiol. 1988 Sep(9):16-9.
Antigens have been obtained from standard Listeria strains 1 and 4a by alcohol precipitation, sonication and multiple freezing and thawing. From these antigens protein fractions, immunologically identical as shown by the precipitation test and immunoelectrophoresis and similar in their chemical composition, have been isolated. The immunogenic properties of the fractions are determined by the polysaccharide-protein complex represented by amino acids (proline, histidine, lysine) and monosaccharides (ribose, glucose, arabinose), common for the fractions of three antigens. In experimental studies on mice and rabbits the protein fraction has been shown to produce a protective effect. After receiving this fraction in three injections and the subsequent challenge with the suspension of Listeria culture in a dose of 500 million cells, the animals have been found to develop no Listeria infection. After autoclaving the protein fraction looses its immunogenic properties, but retains the specificity of chemical groups and produces an allergenic effect as shown in experiments on guinea pigs and rabbits.
通过酒精沉淀、超声处理以及多次冻融从标准李斯特菌菌株1和4a中获取了抗原。从这些抗原中分离出了蛋白质组分，沉淀试验和免疫电泳显示它们在免疫上相同，且化学组成相似。这些组分的免疫原性由多糖 - 蛋白质复合物决定，该复合物由氨基酸（脯氨酸、组氨酸、赖氨酸）和单糖（核糖、葡萄糖、阿拉伯糖）组成，这三种抗原的组分都有这些物质。在对小鼠和兔子的实验研究中，已证明该蛋白质组分具有保护作用。在三次注射该组分并随后用剂量为5亿个细胞的李斯特菌培养物悬液进行攻击后，发现动物未发生李斯特菌感染。蛋白质组分经高压灭菌后失去其免疫原性，但保留了化学基团的特异性，并在豚鼠和兔子的实验中显示出产生过敏反应的作用。
