Neto Edilson Martins Rodrigues, Valadas Lídia Audrey Rocha, Lobo Patrícia Leal Dantas, Fernandes Analice Mendes Barreto, da Cruz Fonseca Said Gonçalves, Fechine Francisco Vagnaldo, Júnior Francisco Josimar Girão, Bandeira Mary Anne Medeiros, de França Fonteles Marta Maria
Postgraduate Program in Drug Development, Pharmacy, Dentistry and Nursing College, Federal University of Ceara, Fortaleza, Brazil.
Clinical Pharmacology Unit, Department of Pharmacology, School of Medicine, Federal University of Ceara, Fortaleza, Brazil.
Recent Pat Biotechnol. 2020;14(1):41-48. doi: 10.2174/1872208313666190826145453.
Early childhood caries is a sugar-dependent disease with multifactorial modulating factors affecting deciduous dentition. It is defined as the presence of at least one decayed tooth, absence of a tooth due to caries or the existence of a temporary restoration in a tooth in a child between zero and 71 months of age. No BRP varnish was found in intellectual property banks, therefore it was registered and deposited with patent number BR1020160190142.
The objective of this study was to evaluate the dose-response concentration of alcoholic extract of Brazilian red propolis (BRP), in the form of dental varnish, against Streptococcus mutans (S. mutans) in children.
Twenty-four children, aged between 36 and 71 months, of both genders and without caries, were selected to participate in this pilot study and grouped randomly into four groups to receive different concentrations of BRP varnish (1%, 2.5%, 5% and 10%). The varnish was applied to the surface of all second deciduous molars. The antimicrobial activity was observed in saliva, which was collected in two phases: before applying the BRP varnish and after use.
There was microbiological reduction of S. mutans in the oral cavity of the children in all the tested concentrations. The highest percentage reduction of S. mutans was observed at the concentration of 2.5% (P = 0.0443).
The BRP extract in the form of dental varnish has antimicrobial activity against S. mutans and constitutes a possible alternative in the prevention of dental caries.
幼儿龋齿是一种依赖糖分的疾病，有多种影响乳牙列的调节因素。它被定义为年龄在0至71个月的儿童中至少有一颗龋齿、因龋齿导致牙齿缺失或牙齿有临时修复体。在知识产权库中未发现巴西红蜂胶（BRP）清漆，因此它已注册并获得专利号BR1020160190142。
本研究的目的是评估牙科清漆形式的巴西红蜂胶（BRP）酒精提取物对儿童变形链球菌（S. mutans）的剂量反应浓度。
选取24名年龄在36至71个月之间、无龋齿的男女儿童参与这项初步研究，并随机分为四组，接受不同浓度的BRP清漆（1%、2.5%、5%和10%）。将清漆应用于所有第二乳磨牙的表面。在两个阶段收集唾液以观察抗菌活性：在应用BRP清漆之前和使用之后。
在所有测试浓度下，儿童口腔中的变形链球菌均有微生物学减少。在2.5%的浓度下观察到变形链球菌减少的百分比最高（P = 0.0443）。
牙科清漆形式的BRP提取物对变形链球菌具有抗菌活性，是预防龋齿的一种可能替代方法。
