人脐带血来源的CD34+造血干细胞和祖细胞的全髓系分化
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells.
作者信息
Bapat Aditi, Keita Nakia, Sharma Shalini
机构信息
Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona.
Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona;
出版信息
J Vis Exp. 2019 Aug 9(150). doi: 10.3791/59836.
Abstract
Ex vivo differentiation of human hematopoietic stem cells is a widely used model for studying hematopoiesis. The protocol described here is for cytokine induced differentiation of CD34 hematopoietic stem and progenitor cells to the four myeloid lineage cells. CD34 cells are isolated from human umbilical cord blood and co-cultured with MS-5 stromal cells in the presence of cytokines. Immunophenotypic characterization of the stem and progenitor cells, and the differentiated myeloid lineage cells are described. Using this protocol, CD34 cells may be incubated with small molecules or transduced with lentiviruses to express myeloid disease mutations to investigate their impact on myeloid differentiation.
摘要
人造血干细胞的体外分化是研究造血作用广泛使用的模型。此处描述的方案是用于细胞因子诱导CD34造血干细胞和祖细胞分化为四种髓系谱系细胞。CD34细胞从人脐带血中分离出来，并在细胞因子存在的情况下与MS-5基质细胞共培养。描述了干细胞、祖细胞以及分化的髓系谱系细胞的免疫表型特征。使用该方案，CD34细胞可以与小分子一起孵育或用慢病毒转导以表达髓系疾病突变，从而研究它们对髓系分化的影响。
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