Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China.
Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China.
J Insect Physiol. 2019 Oct;118:103934. doi: 10.1016/j.jinsphys.2019.103934. Epub 2019 Aug 23.
In insects, nutrition and hormones coordinately regulate lifespan and reproduction, which might affect each other. We here investigated how nutrition, insulin, and juvenile hormone (JH) signal genes affect lifespan and reproduction in the German cockroach, Blattella germanica, a serious urban pest throughout the world. Starvation as well as altering insulin and nutrition signal genes by RNA interference (RNAi) knockdown of the expression of either positive or negative components in the two pathways simultaneously reduced lifespan and ootheca number of the mated female cockroaches. Meanwhile, depletion of the JH receptor Methoprene-tolerant (Met), but not kruppel homolog 1 (Kr-h1) that predominately transduces JH signaling to prevent metamorphosis, significantly reduced the two parameters. Moreover, suppressing the expression of several reproduction-related genes, including doublesex (Dsx), vitellogenin (Vg), and the Vg receptor (VgR), had little yet various effects on lifespan; nevertheless, it is likely that there are some reproduction-independent mating factors reducing lifespan. In conclusion, through blocking lifespan and reproduction in a simultaneous manner, the alteration of insulin and nutrient signal gene expression or the depletion of Met might provide a great potential for controlling the German cockroach.
在昆虫中，营养和激素协同调节寿命和繁殖，这两者可能会相互影响。在这里，我们研究了营养、胰岛素和保幼激素（JH）信号基因如何影响德国蟑螂（Blattella germanica）的寿命和繁殖，德国蟑螂是全世界一种严重的城市害虫。饥饿以及通过 RNA 干扰（RNAi）同时敲低两条通路中的正、负成分来改变胰岛素和营养信号基因，均会缩短交配雌性蟑螂的寿命和卵荚数量。同时，耗尽 JH 受体 Methoprene-tolerant（Met），而不是主要将 JH 信号转导为防止变态的 kruppel 同源物 1（Kr-h1），会显著降低这两个参数。此外，抑制几个与繁殖相关的基因的表达，包括 doublesex（Dsx）、卵黄原蛋白（Vg）和 Vg 受体（VgR），对寿命的影响很小，但可能存在一些与繁殖无关的交配因子会降低寿命。总之，通过同时阻断寿命和繁殖，改变胰岛素和营养信号基因的表达或耗尽 Met，可能为控制德国蟑螂提供了巨大的潜力。
