Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
Fish Shellfish Immunol. 2019 Oct;93:1084-1092. doi: 10.1016/j.fsi.2019.08.055. Epub 2019 Aug 23.
As a family of negatively feedback regulating factors, the suppressor of cytokine signaling (SOCS) can depress cytokine signal transduction, and eventually modulate growth, development, differentiation, and immune response. In the present study, a SOCS homologue (designated as CgSOCS6) was identified from oyster Crassostrea gigas. The open reading frame of CgSOCS6 cDNA was of 1167 bp encoding a peptide of 388 amino acid residues with a central Src homology 2 (SH2) domain, a conserved C-terminal SOCS box, and a nucleus localization sequence (NLS) in its N-terminus. The deduced amino acid sequence of CgSOCS6 shared 37.9-45.5% similarity with other SOCS6/7 family members. In the unrooted phylogenetic tree, CgSOCS6 was clustered with EsSOCS6 from Chinese mitten crab Eriocheir sinensis and assigned into the SOCS6/7 group. The mRNA transcripts of CgSOCS6 were constitutively distributed in all the tested tissues, with the highest level in hemocytes. After lipopolysaccharide (LPS) stimulation, the mRNA expression of CgSOCS6 in hemocytes was significantly up-regulated to the highest level at 6 h (8.48-fold compared to the control group, p < 0.01), and then kept at a relatively higher level from 12 h to 72 h. CgSOCS6 protein could be translocated into the hemocyte nucleus after LPS stimulation. The mRNA expressions of interleukin 17-4 (CgIL17-4), CgIL17-5, and defensin (CgDefh1) in the hemocytes of CgSOCS6-knockdown oysters increased significantly (2.55-fold, 2.68-fold, 4.68-fold of that in EGFP-RNAi oysters, p < 0.05, p < 0.05, p < 0.001, respectively) after LPS stimulation. These findings suggested that CgSOCS6 was involved in the oyster immune response by regulating the expressions of CgIL17-4, CgIL17-5, and CgDefh1.
作为负反馈调节因子家族的一员，细胞因子信号转导抑制因子（SOCS）可以抑制细胞因子信号转导，最终调节生长、发育、分化和免疫反应。本研究从牡蛎（Crassostrea gigas）中鉴定出一种 SOCS 同源物（命名为 CgSOCS6）。CgSOCS6 cDNA 的开放阅读框为 1167bp，编码一个由 388 个氨基酸残基组成的肽，其中央具有 Src 同源 2（SH2）结构域、保守的 C 末端 SOCS 盒和 N 末端核定位序列（NLS）。CgSOCS6 的推导氨基酸序列与其他 SOCS6/7 家族成员具有 37.9-45.5%的相似性。在无根系统发育树中，CgSOCS6 与中华绒螯蟹（Eriocheir sinensis）的 EsSOCS6 聚类，并归入 SOCS6/7 组。CgSOCS6 的 mRNA 转录本在所有检测的组织中均呈组成型分布，在血细胞中表达水平最高。在脂多糖（LPS）刺激后，血细胞中 CgSOCS6 的 mRNA 表达水平在 6 小时（与对照组相比，8.48 倍，p<0.01）上调至最高水平，然后在 12 小时至 72 小时保持相对较高水平。LPS 刺激后，CgSOCS6 蛋白可被转运到血细胞核内。在 CgSOCS6 敲低牡蛎的血细胞中，白细胞介素 17-4（CgIL17-4）、CgIL17-5 和防御素（CgDefh1）的 mRNA 表达显著增加（与 EGFP-RNAi 牡蛎相比，分别为 2.55 倍、2.68 倍和 4.68 倍，p<0.05，p<0.05，p<0.001）。这些发现表明，CgSOCS6 通过调节 CgIL17-4、CgIL17-5 和 CgDefh1 的表达参与牡蛎的免疫反应。
