Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian, 116023, China.
Fish Shellfish Immunol. 2020 Feb;97:554-563. doi: 10.1016/j.fsi.2019.12.080. Epub 2019 Dec 27.
The activator protein-1 (AP-1) plays an important role in inducing the immune effector production in response to cellular stress and bacterial infection. In the present study, an AP-1 was identified from Pacific oyster Crassostrea gigas (designed as CgAP-1) and its function was investigated in response against lipopolysaccharide (LPS) stimulation. CgAP-1 was consisted of 290 amino acids including a Jun domain and a basic region leucine zipper (bZIP) domain. CgAP-1 shared 98.6% similarities with ChAP-1 from oyster C. hongkongensis, and assigned into the branch of invertebrates in the phylogenetic tree. The mRNA transcripts of CgAP-1 gene were detected in all tested tissues with highest expression level in hemocytes, especially in granulocytes. The mRNA expression level of CgAP-1 gene in hemocytes was significantly up-regulated (8.53-fold of that in PBS group, p < 0.01) at 6 h after LPS stimulation. CgAP-1 protein could be translocated into the nucleus of oyster hemocytes after LPS stimulation. The mRNA transcripts of interleukin17s (CgIL17-4 and CgIL17-5) in the hemocytes of CgAP-1-RNAi oysters decreased significantly at 24 h after LPS stimulation, which were 0.37-fold (p < 0.05) and 0.17-fold (p < 0.01) compared with that in EGFP-RNAi oysters, respectively. The results suggested that CgAP-1 played an important role in the immune response of oyster by regulating the expression of CgIL17s.
激活蛋白-1 (AP-1) 在细胞应激和细菌感染时诱导免疫效应产物的产生中发挥重要作用。本研究从太平洋牡蛎（Crassostrea gigas）中鉴定出一个 AP-1（命名为 CgAP-1），并研究了其在应对脂多糖（LPS）刺激时的功能。CgAP-1 由 290 个氨基酸组成，包括一个 Jun 结构域和一个碱性亮氨酸拉链（bZIP）结构域。CgAP-1 与香港牡蛎（C. hongkongensis）的 ChAP-1 相似度为 98.6%，在系统发育树中属于无脊椎动物分支。CgAP-1 基因的 mRNA 转录本在所有检测组织中均有表达，在血细胞中表达水平最高，尤其是在粒细胞中。在 LPS 刺激后 6 小时，血细胞中 CgAP-1 基因的 mRNA 表达水平显著上调（与 PBS 组相比，上调 8.53 倍，p<0.01）。LPS 刺激后，CgAP-1 蛋白可被转运到牡蛎血细胞的核内。在 CgAP-1-RNAi 牡蛎的血细胞中，白细胞介素 17s（CgIL17-4 和 CgIL17-5）的 mRNA 转录本在 LPS 刺激后 24 小时显著下降，分别为 EGFP-RNAi 牡蛎的 0.37 倍（p<0.05）和 0.17 倍（p<0.01）。结果表明，CgAP-1 通过调节 CgIL17s 的表达在牡蛎的免疫反应中发挥重要作用。
