Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao East Rd., Taipei 106, Taiwan.
Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao East Rd., Taipei 106, Taiwan.
Ultrason Sonochem. 2019 Nov;58:104622. doi: 10.1016/j.ultsonch.2019.104622. Epub 2019 May 31.
To explore a novel and multi-layer based graphene oxide covered zinc oxide nanoflower (ZnO NFs@GOS) as a modified electrode materials by sonochemical technique (40 kHz, 300 W). Herein, novel nanocomposite is successfully characterized by various characterization analysis (FESEM, HRTEM, XRD, XPS and (EIS) electrochemical impedance spectroscopy) and employed as high sensitive modified electrode (ZnO NFs@GOS nanocomposite) for the electrochemical determination of biomarker. 8-hydroxy-2'-deoxyguanosine (8-HDG) is one of the important cancer and oxidative stress biomarker. The results demonstrated that the ZnO NFs@GOS modified SPCE reveal well-defined electro-oxidation peak at 0.36 V (vs. Ag/AgCl). The high sensitive properties of the optimized flower like modified electrode are because of the excellent synergistic effect of the ZnO flower and the graphene oxide nanosheets, as evidenced by a superior bio-sensing performance. The nanocomposite fabricated modified biosensor was facilitating the analysis of 8-HDG in the concentration ranges of 0.05-536.5 µM with a low detection limit is 8.67 nM. The ZnO NFs@GOS modified sensor can also employed for the determination of 8-HDG in human urine samples, promising its application towards the quantification of cancer biomarker in biological samples.
采用超声化学技术（40kHz，300W）探索了一种新型的、基于多层的氧化石墨烯覆盖氧化锌纳米花（ZnO NFs@GOS）作为修饰电极材料。在此，通过各种特征分析（FESEM、HRTEM、XRD、XPS 和（EIS）电化学阻抗谱）成功地对新型纳米复合材料进行了表征，并将其用作高灵敏修饰电极（ZnO NFs@GOS 纳米复合材料），用于生物标志物的电化学测定。8-羟基-2'-脱氧鸟苷（8-HDG）是一种重要的癌症和氧化应激生物标志物。结果表明，在 ZnO NFs@GOS 修饰的 SPCE 上，在 0.36V（相对于 Ag/AgCl）处显示出明确的电氧化峰。优化后的花状修饰电极具有较高的灵敏度，这是由于 ZnO 花和氧化石墨烯纳米片的协同效应非常好，这一点可以从优越的生物传感性能得到证明。所制备的纳米复合材料修饰的生物传感器有利于在 0.05-536.5µM 的浓度范围内分析 8-HDG，检测限低至 8.67nM。ZnO NFs@GOS 修饰的传感器还可用于测定人尿样中的 8-HDG，有望应用于生物样品中癌症生物标志物的定量分析。
