School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China.
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China.
Talanta. 2019 Dec 1;205:120021. doi: 10.1016/j.talanta.2019.06.021. Epub 2019 Jun 15.
Herein, we figured out a novel ratiometric fluorescent probe based on Janus silica nanoparticles (SiO NPs) where fluorescein isothiocyanate (FITC) and rhodamine B (RB) were asymmetrically functionalized for intracellular pH detection. With this unique strategy, the probe exhibited dual emission bands at 530 and 580 nm upon excitation at a single wavelength of 488 nm. The pH titrations of the probe showed a remarkable increase in ratiometric intensities ratio (I/I) when the pH was switched from 3.0 to 8.0 with a acid dissociation constant (pKa) value of 6.64. The probe was successfully applied to imaging and detection of pH in living cells without noticeable cytotoxicity, indicating that it is a promising candidate for bioanalysis and bioimaging.
在这里，我们设计了一种基于手性二氧化硅纳米粒子（SiO NPs）的新型比率荧光探针，其中异硫氰酸荧光素（FITC）和罗丹明 B（RB）被不对称地功能化，用于细胞内 pH 检测。通过这种独特的策略，探针在单个 488nm 波长激发下显示出 530nm 和 580nm 的双发射带。探针的 pH 滴定显示，当 pH 值从 3.0 切换到 8.0 时，比率强度比（I/I）有显著增加，其酸离解常数（pKa）值为 6.64。该探针成功地应用于活细胞中 pH 的成像和检测，没有明显的细胞毒性，表明它是生物分析和生物成像的有前途的候选者。
