Departamento de Farmacia, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud. Universidad Cardenal Herrera-CEU , Valencia , España.
ESI International Chair@CEU-UCH. Departamento de Matemáticas, Física y Ciencias Tecnológicas. Universidad Cardenal Herrera-CEU , Valencia , España.
Virulence. 2019 Dec;10(1):776-782. doi: 10.1080/21505594.2019.1659663.
Experimentation in mammals is a long and expensive process in which ethical aspects must be considered, which has led the scientific community to develop alternative models such as that of . This model is a cost and time effective option to act as a filter in the drug discovery process. The main limitation of this model is the lack of variety in the solvents used to administer compounds, which limits the compounds that can be studied using this model. Five aqueous (DMSO, MeOH, acetic acid, HCl and NaOH) and four non-aqueous (olive oil, isopropyl myristate, benzyl benzoate and ethyl oleate) solvents was assessed to be used as vehicles for toxicity and antimicrobial activity assays. All the tested solvents were innocuous at the tested concentrations except for NaOH, which can be used at a maximum concentration of 0.5 M. The toxicity of two additional compounds, 5-aminosalicylic acid and DDT, was also assessed. The results obtained allow for the testing of a broader range of compounds using wax moth larvae. This model appears as an alternative to mammal models, by acting as a filter in the drug development process and reducing costs and time invested in new drugs.
哺乳动物实验是一个漫长而昂贵的过程，其中必须考虑到伦理方面的问题，这导致科学界开发了替代模型，如 。这种模型是在药物发现过程中作为筛选器的一种具有成本效益和时间效益的选择。这种模型的主要限制是用于给药化合物的溶剂种类有限，这限制了可以使用该模型研究的化合物种类。评估了五种水性（DMSO、MeOH、乙酸、HCl 和 NaOH）和四种非水性（橄榄油、肉豆蔻异丙酯、苯甲酸苄酯和油酸乙酯）溶剂作为毒性和抗菌活性测定的载体。除了 NaOH 之外，所有测试的溶剂在测试浓度下都是无害的，NaOH 的最大使用浓度为 0.5 M。还评估了另外两种化合物，5-氨基水杨酸和滴滴涕的毒性。所得结果允许使用蜡螟幼虫测试更广泛范围的化合物。该模型通过在药物开发过程中充当筛选器并降低新药投资的成本和时间，成为哺乳动物模型的替代方案。
