Envigo Ltd, Huntingdon, Cambridgeshire, UK.
Biosciences University of Exeter, Exeter, EX4 4QD, UK.
Chemosphere. 2018 May;198:469-472. doi: 10.1016/j.chemosphere.2018.01.175. Epub 2018 Feb 6.
The acute toxicities of 19 chemicals were assessed using G. mellonella larvae. The results obtained were compared against LD50 values derived from in vitro cytotoxicity tests and against in vivo acute oral LD50 values. In general, cell culture systems overestimated the toxicity of chemicals, especially low toxicity chemicals. In contrast, toxicity testing in G. mellonella larvae was found to be a reliable predictor for low toxicity chemicals. For the 9 chemicals tested which were assigned to Globally Harmonised System (GHS) category 5, the toxicity measured in G. mellonella larvae was consistent with their GHS categorisation but cytotoxicity measured in 3T3 or NHK cells predicted 4 out of 9 chemicals as having low toxicity. A more robust assessment of the likely toxicity of chemicals in mammals could be made by taking into account their toxicities in both cell cultures and in G. mellonella larvae.
使用家蚕幼虫评估了 19 种化学物质的急性毒性。将获得的结果与体外细胞毒性试验得出的 LD50 值以及体内急性口服 LD50 值进行了比较。一般来说，细胞培养系统高估了化学物质的毒性，尤其是低毒性化学物质的毒性。相比之下，在家蚕幼虫中的毒性测试被发现是低毒性化学物质的可靠预测指标。对于测试的 9 种被归类为全球协调系统（GHS）第 5 类的化学物质，在家蚕幼虫中测量的毒性与其 GHS 分类一致，但在 3T3 或 NHK 细胞中测量的细胞毒性预测 9 种化学物质中有 4 种具有低毒性。通过同时考虑化学物质在细胞培养物和家蚕幼虫中的毒性，可以对哺乳动物中化学物质的潜在毒性进行更稳健的评估。
