Department of Molecular Physics, Yerevan State University, Yerevan, Armenia.
J Biomol Struct Dyn. 2020 Aug;38(12):3489-3495. doi: 10.1080/07391102.2019.1660217. Epub 2019 Sep 9.
The present paper is an overview of studying of DNA-porphyrin interactions using viscometry in combination with the spectroscopic methods. It was shown, that when porphyrins interact with DNA as an outside binder, the interaction mode and intensity does not depend on metal center, peripheral substituent's and their positions on pyridylic ring. In case of planar porphyrins, the binding type is mainly determined by type of peripheral substituent's and their position on the pyridylic ring. Currently, viscometry is widely used to study the interaction of porphyrins with DNA as an adjunct to other methods. Due to high accuracy and maximum sensitivity to changes in the size and shape of macromolecules, it is recommended to use viscometry as the cogent method for studying the interaction of small molecules with DNA, especially if intercalation is expected using other methods if necessary to confirm the results obtained. AbbreviationsDNAdeoxyribonucleic acidHTAllPyP4-tetra-(4N-allylpyridyl) porphyrinHTAllPyP3-tetra-(3N-allylpyridyl) porphyrinHTHOEtPyP4-tetra-(4N-hydroxyethylpyridyl) porphyrinHTHOEtPyP3-tetra-(3N-hydroxyethylpyridyl) porphyrinUV/VIS spectrophotometryultraviolet-visible spectrophotometryCD spectroscopycircular dichroism spectroscopyCommunicated by Ramaswamy H. Sarma.
本文综述了利用粘度法结合光谱法研究 DNA-卟啉相互作用。结果表明，当卟啉作为外部配体与 DNA 相互作用时，相互作用模式和强度与金属中心、外围取代基及其在吡啶环上的位置无关。对于平面卟啉，结合类型主要由外围取代基的类型及其在吡啶环上的位置决定。目前，粘度法广泛用于研究卟啉与 DNA 的相互作用，作为其他方法的补充。由于粘度法具有高精度和对大分子尺寸和形状变化的最大灵敏度，建议将其用作研究小分子与 DNA 相互作用的有力方法，特别是如果预期使用其他方法进行嵌入，则有必要使用粘度法来确认获得的结果。缩写词DNA 脱氧核糖核酸 HTAllPyP4-四-（4N-烯丙基吡啶基）卟啉 HTAllPyP3-四-（3N-烯丙基吡啶基）卟啉 HTHOEtPyP4-四-（4N-羟乙基吡啶基）卟啉 HTHOEtPyP3-四-（3N-羟乙基吡啶基）卟啉 UV/VIS 分光光度法 紫外可见分光光度法 CD 光谱学 圆二色光谱学 由 Ramaswamy H. Sarma 传达。
