Department of Intensive Care Unit, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, Hubei, China.
Teaching and Research Division of Surgical Medicine, Hubei College of Chinese Medicine, Jingzhou, China.
J Cell Biochem. 2020 Jan;121(1):779-787. doi: 10.1002/jcb.29323. Epub 2019 Aug 26.
Lung cancer is an significant cause of death worldwide, and non-small-cell lung cancer (NSCLC) is the most common type of lung cancer. MicroRNAs (miRNAs) have been identified to play key roles in NSCLC development. Recently, it has been reported that miR-605-5p is a cancer-related miRNA in several types of tumors. In this study, we study the role of miR-605-5p in NSCLC cells. We find that miR-605-5p is upregulated in NSCLC cells. Overexpression of miR-605-5p significantly promotes lung cancer invasion and migration in H460 and H1299 cells. Besides this, miR-605-5p also promotes lung cancer cell carcinoma proliferation and metastasis in vivo. However, downregulation of miR-605-5p inhibits cell invasion and migration by inhibiting lung cancer cell carcinoma proliferation and metastasis. In addition, the luciferase report assay identifies 3'-untranslated region tumor necrosis factor α-induced protein 3 (TNFAIP3) as a target of miR-605-5p. Silencing of TNFAIP3 promotes invasion and proliferation in lung cancer. In addition, the knockdown of TNFAIP3 restores the significant decrease in invasion and proliferation in miR-605-5p-inhibitor-transfected lung cancer cells. In conclusion, miR-605-5p promotes invasion and proliferation by targeting TNFAIP3 in NSCLC, and may provide possible biomarkers for NSCLC therapy.
肺癌是全球范围内重要的死亡原因之一，非小细胞肺癌（NSCLC）是最常见的肺癌类型。microRNAs（miRNAs）已被确定在 NSCLC 的发展中发挥关键作用。最近，有研究报道 miR-605-5p 是几种类型肿瘤中的一种癌症相关 miRNA。在本研究中，我们研究了 miR-605-5p 在 NSCLC 细胞中的作用。我们发现 miR-605-5p 在 NSCLC 细胞中上调。miR-605-5p 的过表达显著促进了 H460 和 H1299 细胞中的肺癌侵袭和迁移。除此之外，miR-605-5p 还促进了体内肺癌细胞的增殖和转移。然而，下调 miR-605-5p 通过抑制肺癌细胞的增殖和转移抑制了细胞侵袭和迁移。此外，荧光素酶报告试验鉴定出 3'-非翻译区肿瘤坏死因子α诱导蛋白 3（TNFAIP3）是 miR-605-5p 的靶标。TNFAIP3 的沉默促进了肺癌的侵袭和增殖。此外，TNFAIP3 的敲低恢复了 miR-605-5p 抑制剂转染的肺癌细胞中侵袭和增殖的显著减少。总之，miR-605-5p 通过靶向 TNFAIP3 促进 NSCLC 的侵袭和增殖，可能为 NSCLC 的治疗提供潜在的生物标志物。
