Ruyani Aceng, Sinta Barbara Desbi, Anansyah Fiqih, Putri Sylvia Rianissa, Sundaryono Agus
Department of Biology Education, Bengkulu University, Jalan Raya Kandang Limun, Bengkulu 38371, Indonesia.
Graduate School of Science Education, Bengkulu University, Jalan Raya Kandang Limun, Bengkulu 38371, Indonesia.
J Tradit Complement Med. 2018 Sep 25;9(4):285-296. doi: 10.1016/j.jtcme.2017.08.005. eCollection 2019 Oct.
This study intended to investigate the therapeutic effect of ethanolic extract of leaves (E2TL) against paracetamol (PC)-induced hepatotoxicity (PCIH) in mice (). PCIH were generated using daily 250 mg/kg body weight (bw) PC administration by gavage for seven days, and then daily 27.5; 55.0; 82.5; 110.0; or 220.0 mg/kg bw E2TL were treated by gavage for seven or fourteen days. Meanwhile, the controls were given solvent only in the same manner. Mortality, blood glucose, and condition (color, weight, volume) of the livers were observed on day 15 (D15). Serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SG0T) were examined on D15, D22, and D30, and then malondialdehyde (MDA) was determined on D15. Results of this study revealed that on D15, the dosage of 110.0 mg/kg bw E2TL most effectively decreased MDA due to PCIH, from 6.78 ± 1.70 μmol/L to 3.45 ± 0.43 μmol/L, approaching the control condition (2.45 ± 0.05 μmol/L). PC administration was really toxic dosage and caused 13.3 % mortality. Blood glucose, weight, and volume of the liver decreased as the effect of PC administration, and then 220.0 mg/kg bw E2TL treatment could recover the condition as well as the controls. Color of the liver indicated a similar recovery by E2TL treatment. SGPT and SG0T increased significantly by PC administration, and this PCIH facts could be recovered gradually near the controls according to the dosages (55.0; 110.0; or 220.0 mg/kg bw) and duration (seven or fourteen days) of E2TL treatment. It could be concluded that E2TL showed therapeutic effect against PCIH in .
本研究旨在探讨叶乙醇提取物（E2TL）对小鼠扑热息痛（PC）诱导的肝毒性（PCIH）的治疗效果。通过每天经口灌胃给予250mg/kg体重的PC，持续7天来诱导PCIH，然后每天经口灌胃给予27.5、55.0、82.5、110.0或220.0mg/kg体重的E2TL，持续7天或14天。同时，对照组以相同方式仅给予溶剂。在第15天（D15）观察死亡率、血糖以及肝脏的状况（颜色、重量、体积）。在D15、D22和D30检测血清谷丙转氨酶（SGPT）和血清谷草转氨酶（SGOT），然后在D15测定丙二醛（MDA）。本研究结果显示，在D15，110.0mg/kg体重的E2TL剂量最有效地降低了PCIH导致的MDA，从6.78±1.70μmol/L降至3.45±0.43μmol/L，接近对照状态（2.45±0.05μmol/L）。PC给药剂量确实有毒，导致13.3%的死亡率。血糖、肝脏重量和体积因PC给药而降低，然后220.0mg/kg体重的E2TL治疗可使状况恢复至与对照组相同。肝脏颜色表明E2TL治疗有类似的恢复情况。PC给药使SGPT和SGOT显著升高，根据E2TL治疗的剂量（55.0、110.0或220.0mg/kg体重）和持续时间（7天或14天），这种PCIH情况可逐渐恢复至接近对照组。可以得出结论，E2TL对小鼠的PCIH显示出治疗效果。
