Graduate School of Science Education, Bengkulu University, Bengkulu, Indonesia.
Department of Biology Education, Bengkulu University, Bengkulu, Indonesia.
PLoS One. 2021 Mar 10;16(3):e0247467. doi: 10.1371/journal.pone.0247467. eCollection 2021.
This study aimed to investigate the effectiveness of leaf ethanolic extract of Etlingera hemisphaerica (LE3H) in reducing defects in fetal anatomy and endochondral ossification in mice induced by HgCl2 during the post-implantation period. Pregnant mice were divided into four groups, each consisting of 10 dams, and received drink and food ad libitum. The first group was administered LE3H (E1), the second one HgCl2 (E2), the third one HgCl2+LE3H (E3), and the fourth was control (E0), administered double-distilled water only. HgCl2 (5 mg/kg bw) was administrated by injection intraperitoneally on gestation day (GD)9 and LE3H (0.39 mg/g bw) was administered by gavage on GD10. The treated and control animals were killed by cervical dislocation on GD18, dissected, and the morphologically normal living fetuses (MNLF) were collected. The MNLF of E0, E1, E2, and E3 from 5 dams were fixed with Bouin solution, and observed using the free hand razor blade technique for soft tissue examination. The remaining MNLF were fixed with 96% ethanol, and then stained with Alizarin Red S and Alcian Blue for ossification examination. Index of length of ossified part (ILOP) of humerus, index of width of ossified part (IWOP) of humerus, ILOP of femur, and IWOP of femur were calculated. E2 had higher cases of anatomical defects (74,6%) than E3 (48.9%), E1 (15.0%), and E0 (0%). E2 had humerus IWOP of 0.82±0.03, which was significantly lower than that of E0 (0.89±0.04) and E1 (0.89±0.03), while that of E1 and E0 was not significantly different from each other. Meanwhile, IWOP in E3 (0.88±0.03) was significantly higher than that in E2, but not different from that in E1 and E0. Thus, LE3H mitigated defects in fetal anatomy and endochondral ossification induced by HgCl2 in mice.
本研究旨在探讨 Etlingera hemisphaerica 叶乙醇提取物（LE3H）在减少汞氯化物（HgCl2）在着床后时期诱导的小鼠胎儿解剖和软骨内骨化缺陷方面的有效性。将怀孕的小鼠分为四组，每组由 10 只母鼠组成，自由摄取饮料和食物。第一组给予 LE3H（E1），第二组给予 HgCl2（E2），第三组给予 HgCl2+LE3H（E3），第四组为对照组（E0），仅给予双蒸水。HgCl2（5mg/kg bw）于妊娠第 9 天通过腹腔注射给药，LE3H（0.39mg/g bw）于妊娠第 10 天通过灌胃给药。用颈椎脱位处死处理和对照动物，解剖，收集形态正常的活胎（MNLF）。从 5 只母鼠的 E0、E1、E2 和 E3 中取出 MNLF 用 Bouin 溶液固定，并用游离刀片技术观察软组织。其余的 MNLF 用 96%乙醇固定，然后用茜素红 S 和阿利新蓝染色进行骨化检查。肱骨骨化部分长度指数（ILOP）、肱骨骨化部分宽度指数（IWOP）、股骨 ILOP 和股骨 IWOP 进行计算。E2 的解剖缺陷病例（74.6%）高于 E3（48.9%）、E1（15.0%）和 E0（0%）。E2 的肱骨 IWOP 为 0.82±0.03，明显低于 E0（0.89±0.04）和 E1（0.89±0.03），而 E1 和 E0 之间无显著差异。同时，E3 的 IWOP（0.88±0.03）明显高于 E2，但与 E1 和 E0 无差异。因此，LE3H 减轻了 HgCl2 诱导的小鼠胎儿解剖和软骨内骨化缺陷。
