Kakadia Nimisha, Patel Payal, Deshpande Shrikalp, Shah Gaurang
Department of Pharmacology and Pharmacy Practice, K. B. Institute of Pharmaceutical Education and Research, GH-6, Sector-23, Gandhinagar, 382023, Gujarat, India.
J Tradit Complement Med. 2018 Oct 11;9(4):336-345. doi: 10.1016/j.jtcme.2018.03.001. eCollection 2019 Oct.
The clinical management of PCOS is multifaceted but often unsatisfactory. The aim of the current study is to evaluate the effect of L. in the letrozole-induced polycystic ovarian syndrome. Female Sprague-Dawley rats were divided into six groups, each containing 6 animals. Group I (Control) daily received 1% carboxymethylcellulose (CMC) suspension as a vehicle control. Letrozole (1 mg/kg) was administered per orally (p.o) for a period of 21 days for the induction of PCOS in Group II to VI. PCOS induced animals were treated with aqueous (Group III - 200 mg/kg and IV- 400 mg/kg) and hydroalcoholic extract (Group V- 200 mg/kg and VI- 400 mg/kg) of up to 66 days using 0.5% w/v CMC as the vehicle. Body weight and estrous cycle phase were measured every day. Blood samples were collected on 0, 21 and 66 days for the measurement of fasting blood glucose, lipid profile, LH, FSH and hormonal level. Oral glucose tolerance test was performed to study insulin resistance effect. Toxicity markers; SGOT, SGPT, and creatinine also measured at the end of the study. The administration of Letrozole led to an abnormality in serum sex steroid profile, lipid profile, glucose and estrous cycle. It was able to successfully exert its protective effect by restoring parameters to the normal level and disappearance of cysts in ovaries. This can be attributed to phyto-components present in the extract. The aqueous and hydro-alcoholic extracts of seeds of showed significant amelioration of Letrozole induced PCOS.
多囊卵巢综合征（PCOS）的临床管理涉及多个方面，但往往不尽人意。本研究的目的是评估[植物名称未给出]在来曲唑诱导的多囊卵巢综合征中的作用。将雌性Sprague-Dawley大鼠分为六组，每组6只动物。第一组（对照组）每天接受1%羧甲基纤维素（CMC）悬浮液作为载体对照。第二组至第六组口服给予来曲唑（1mg/kg），持续21天以诱导PCOS。诱导出PCOS的动物分别用[植物名称未给出]的水提取物（第三组 - 200mg/kg和第四组 - 400mg/kg）和水醇提取物（第五组 - 200mg/kg和第六组 - 400mg/kg）进行治疗，持续66天，使用0.5% w/v CMC作为载体。每天测量体重和发情周期阶段。在第0、21和66天采集血样，用于测量空腹血糖、血脂谱、促黄体生成素（LH）、促卵泡生成素（FSH）和激素水平。进行口服葡萄糖耐量试验以研究胰岛素抵抗作用。在研究结束时还测量了毒性标志物；谷草转氨酶（SGOT）、谷丙转氨酶（SGPT）和肌酐。来曲唑的给药导致血清性类固醇谱、血脂谱、血糖和发情周期出现异常。它能够通过将参数恢复到正常水平以及卵巢囊肿消失来成功发挥其保护作用。这可归因于提取物中存在的植物成分。[植物名称未给出]种子的水提取物和水醇提取物对来曲唑诱导的PCOS有显著改善作用。
