Doh Jung H, Moore Andrew B, Çelen İrem, Moore Michael T, Sabanayagam Chandram R
University of Delaware, Delaware Biotechnology Institute, Newark, DE, USA.
University of Delaware, Department of Biological Sciences, Newark, DE, USA.
J Biol Methods. 2016 Jun 10;3(2):e44. doi: 10.14440/jbm.2016.109. eCollection 2016.
We present the WormPharm, an automated microfluidic platform that utilizes an axenic medium to culture . The WormPharm is capable of sustaining for extended periods, while recording worm development and growth with high temporal resolution ranging from seconds to minutes over several days to months. We demonstrate the utility of the device to monitor growth in the presence of varying doses of nicotine and alcohol. Furthermore, we show that cultured in the WormPharm are amendable for high-throughput genomic assays, chromatin-immunoprecipitation followed by next generation sequencing, and confirm that nematodes grown in monoxenic and axenic cultures exhibit genetic modifications that correlate with observed phenotypes. The WormPharm is a powerful tool for analyzing the effects of chemical, nutritional and environmental variations on organism level responses in conjunction with genome-wide changes in .
我们展示了WormPharm，这是一个利用无菌培养基进行培养的自动化微流控平台。WormPharm能够长时间维持培养，同时以从秒到分钟的高时间分辨率记录蠕虫在数天至数月内的发育和生长情况。我们展示了该设备在监测不同剂量尼古丁和酒精存在下蠕虫生长方面的效用。此外，我们表明在WormPharm中培养的蠕虫适用于高通量基因组分析，即染色质免疫沉淀后进行下一代测序，并证实单菌培养和无菌培养中生长的线虫表现出与观察到的表型相关的基因修饰。WormPharm是一种强大的工具，可用于结合全基因组变化分析化学、营养和环境变化对生物体水平反应的影响。
