Center for Bioinformatics and Computational Biology, University of Delaware, Newark, DE 19711, USA.
Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.
Cells. 2023 Jan 10;12(2):270. doi: 10.3390/cells12020270.
Given the growing interest in human exploration of space, it is crucial to identify the effects of space conditions on biological processes. Here, we analyze the transcriptomic response of to simulated microgravity and observe the maintained transcriptomic response after returning to ground conditions for four, eight, and twelve days. We show that 75% of the simulated microgravity-induced changes on gene expression persist after returning to ground conditions for four days while most of these changes are reverted after twelve days. Our results from integrative RNA-seq and mass spectrometry analyses suggest that simulated microgravity affects longevity-regulating insulin/IGF-1 and sphingolipid signaling pathways. Finally, we identified 118 genes that are commonly differentially expressed in simulated microgravity- and space-exposed worms. Overall, this work provides insight into the effect of microgravity on biological systems during and after exposure.
鉴于人们对人类太空探索的兴趣日益浓厚，确定空间条件对生物过程的影响至关重要。在这里，我们分析了 对模拟微重力的转录组反应，并观察了返回地面条件后四天、八天和十二天的转录组反应。我们表明，在返回地面条件四天后，75%的模拟微重力诱导的基因表达变化仍然存在，而在十二天后，这些变化中的大多数都得到了逆转。我们从整合 RNA-seq 和质谱分析的结果表明，模拟微重力会影响调节寿命的胰岛素/IGF-1 和鞘脂信号通路。最后，我们鉴定了 118 个在模拟微重力和太空暴露的线虫中共同差异表达的基因。总的来说，这项工作深入了解了微重力在暴露期间和之后对生物系统的影响。
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