Yadav Yogesh Chand
Department of Pharmacology, Faculty of Pharmacy, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, 206130, UP, India.
Heliyon. 2019 Aug 8;5(8):e02247. doi: 10.1016/j.heliyon.2019.e02247. eCollection 2019 Aug.
To investigate effect of cisplatin on biochemical parameter and histology of pancreas and testis in Wistar rats.
Single dose cisplatin (10 mg/kg) was injected by intraperitoneal route in Wistar rats. Blood was withdrawn on 7 day from cisplatin treated rats by retro-orbital sinus for biochemical estimation. Further rats were scarified and dissected out their pancreases and testes for estimation of antioxidant enzymes and histopathological study.
The cisplatin-treated group showed a significantly () increased blood glucose level, Glycosylated hemoglobin in blood on the 7 day as compared to the control group. Whereas cisplatin-treated group showed significantly () increased lipid peroxidation and decreased reduced glutathione, superoxide dismutase, catalase in pancreatic and testicular tissue as compared to the control group. Histopathological sections of the pancreatic tissue showed marked vasoconstriction and micro infiltration were observed however testicular tissue showed degeneration in some somniferous tubules and also greatly depleted of germ cells in cisplatin treated group.
These findings demonstrated that the cisplatin could be induced diabetes and testicular toxicity due to their free radical mediated oxidative stress.
研究顺铂对Wistar大鼠胰腺和睾丸生化参数及组织学的影响。
通过腹腔注射途径给Wistar大鼠单剂量注射顺铂（10mg/kg）。在顺铂处理后的第7天，通过眶后窦从大鼠眼眶取血进行生化指标检测。随后处死大鼠，取出胰腺和睾丸用于抗氧化酶的测定及组织病理学研究。
与对照组相比，顺铂处理组在第7天时血糖水平显著升高，血液中糖化血红蛋白水平显著升高。与对照组相比，顺铂处理组胰腺和睾丸组织中的脂质过氧化显著增加，还原型谷胱甘肽、超氧化物歧化酶、过氧化氢酶含量降低。胰腺组织的组织病理学切片显示有明显的血管收缩和微浸润，而在顺铂处理组中，睾丸组织的一些生精小管出现退化，生殖细胞也大量减少。
这些发现表明，顺铂可因其自由基介导的氧化应激而诱发糖尿病和睾丸毒性。
