新测序的布鲁氏菌流产疫苗株 A19 与另一布鲁氏菌流产疫苗 S19 之间的比较基因组分析。
Comparative genomic analysis between newly sequenced Brucella abortus vaccine strain A19 and another Brucella abortus vaccine S19.
机构信息
Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture/College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot, Inner Mongolia 010031, China.
Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture/College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China.
出版信息
Genomics. 2020 Mar;112(2):1444-1453. doi: 10.1016/j.ygeno.2019.08.015. Epub 2019 Aug 24.
BACKGROUND
Brucellosis is a bacterial disease caused by Brucella infection. Brucella abortus strain A19 is a spontaneously attenuated vaccine strain that has been used in vaccination of cattle against brucellosis. Until now, the physiological and molecular mechanisms of A19 are still unknown.
RESULTS
In this paper, the whole-genome sequence of B. abortus A19 was performed using Illumina Hiseq 4000 and PacBio sequencing technology and comparative genomics analysis were carried out with the whole genome sequences of B. abortus strains S19. This analysis indicated that the two vaccine strains have a high degree of similarity in genomic structure. We further analysis of the difference in genomic structure between A19 and S19. And found some differential genes such as eryC, eryD and eryF. Of the other different proteins between A19 and S19, such as outer membrane protein, 2-isopropylmalate synthase, citramalate synthase, GntR family transcriptional regulator and ABC transporters, no clear effects related to bacterial virulence were found, pending further investigation.
CONCLUSION
The data presented here provide a reasonable basis for designing Brucella vaccines that can be used in other strains.
背景
布鲁氏菌病是由布鲁氏菌感染引起的细菌性疾病。布鲁氏菌 abortus 菌株 A19 是一种自发减毒的疫苗株，已被用于牛布鲁氏菌病的疫苗接种。到目前为止，A19 的生理和分子机制仍不清楚。
结果
在本文中，使用 Illumina Hiseq 4000 和 PacBio 测序技术对 B.abortus A19 的全基因组序列进行了测序，并与 B.abortus 菌株 S19 的全基因组序列进行了比较基因组分析。该分析表明，两种疫苗株在基因组结构上具有高度相似性。我们进一步分析了 A19 和 S19 之间基因组结构的差异。并发现了一些差异基因，如 eryC、eryD 和 eryF。在 A19 和 S19 之间的其他不同蛋白质中，如外膜蛋白、2-异丙基苹果酸合酶、柠檬酸合酶、GntR 家族转录调节剂和 ABC 转运体，没有发现与细菌毒力相关的明显影响，有待进一步研究。
结论
本研究提供的数据为设计可用于其他菌株的布鲁氏菌疫苗提供了合理的依据。
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